Immunologic memory responses induced in BALB/c mice by cross-linked outer membrane extracts of four Salmonella serotypes.

D. A. Kudrna, G. W. Teresa, J. M. Arnzen, K. S. Beard, D. Olson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Outer membrane proteins (OMP), extracted from Salmonella enteritidis, S anatum, S typhimurium, and S infantis, were cross-linked to form a large immunogen (4-OMP-lipopolysaccharide [LPS]). Vaccinations with 4-OMP-LPS dissolved in phosphate-buffered saline solution and 4-OMP-LPS emulsified with muramyl dipeptide were capable of eliciting specific and sustained primary IgM and IgG responses in BALB/c mice, as well as inducing immunologic memory for 130 days. In addition to 4-OMP-LPS-specific responses, substantial IgM and IgG responses specific for each live homologous organism were detected over the 130-day trial. In comparison with vaccination with 4-OMP-LPS dissolved in phosphate-buffered saline solution, responses specific for the antigen or the homologous Salmonella were not markedly increased in mice vaccinated with 4-OMP-LPS emulsified with muramyl dipeptide. Seemingly, cross-linked OMP, without the inclusion of muramyl dipeptide, may have potential as vaccine components and may induce immunologic memory.

Original languageEnglish (US)
Pages (from-to)1199-1205
Number of pages7
JournalAmerican journal of veterinary research
Volume48
Issue number8
StatePublished - Aug 1 1987

ASJC Scopus subject areas

  • veterinary(all)

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