Immunological Methods for Nursing Research: From Cells to Systems

Helena W. Morrison, Charles A. Downs

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Scientists and clinicians frequently use immunological methods (IMs) to investigate complex biological phenomena. Commonly used IMs include immunocytochemistry (IC), enzyme-linked immunosorbent assays (ELISA) and flow cytometry. Each of these methodologies exploits a common principle in IMs -the binding of an antibody to its antigen. Scientists continue to develop new methodologies, such as high-throughput immunohistochemistry (IHC) and in vivo imaging techniques, which exploit antibody-antigen binding, to more accurately answer complex research questions involving single cells up to whole organ systems. The purpose of this paper is to discuss established and evolving IMs and to illustrate the application of these methods to nursing research.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalBiological Research For Nursing
Volume13
Issue number3
DOIs
StatePublished - Jul 1 2011
Externally publishedYes

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Keywords

  • ELISA
  • flow cytometry
  • immunocytochemistry
  • immunology
  • methods

ASJC Scopus subject areas

  • Research and Theory

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