Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; Comparison to immunofluorescence results

Gregory A Bradley, M. B. Calderwood Mays

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17 22 cases. The immunoperoxidase method yielded an overall positive result in 59% ( 5 8 lupus erythematosus, 2 3 pemphigus vulgaris and 6 11 pemphigus foliaceus) versus an overall positive result of 47% for direct immunofluorescence ( 3 5 lupus erythematosus, 2 2 pemphigus vulgaris and 2 10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume26
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

pemphigus (skin disease)
autoantibodies
Pemphigus
skin diseases
Skin Diseases
Autoantibodies
fluorescent antibody technique
Autoimmune Diseases
Fluorescent Antibody Technique
Canidae
Staining and Labeling
dogs
lupus erythematosus
Direct Fluorescent Antibody Technique
skin (animal)
Skin
Lupus Vulgaris
staining
methodology
immunoglobulins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

@article{e1bf4ed5ff5d4779824afbc2559dab0a,
title = "Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; Comparison to immunofluorescence results",
abstract = "Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17 22 cases. The immunoperoxidase method yielded an overall positive result in 59{\%} ( 5 8 lupus erythematosus, 2 3 pemphigus vulgaris and 6 11 pemphigus foliaceus) versus an overall positive result of 47{\%} for direct immunofluorescence ( 3 5 lupus erythematosus, 2 2 pemphigus vulgaris and 2 10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.",
author = "Bradley, {Gregory A} and {Calderwood Mays}, {M. B.}",
year = "1990",
doi = "10.1016/0165-2427(90)90060-6",
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pages = "105--113",
journal = "Veterinary Immunology and Immunopathology",
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TY - JOUR

T1 - Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; Comparison to immunofluorescence results

AU - Bradley, Gregory A

AU - Calderwood Mays, M. B.

PY - 1990

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N2 - Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17 22 cases. The immunoperoxidase method yielded an overall positive result in 59% ( 5 8 lupus erythematosus, 2 3 pemphigus vulgaris and 6 11 pemphigus foliaceus) versus an overall positive result of 47% for direct immunofluorescence ( 3 5 lupus erythematosus, 2 2 pemphigus vulgaris and 2 10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.

AB - Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17 22 cases. The immunoperoxidase method yielded an overall positive result in 59% ( 5 8 lupus erythematosus, 2 3 pemphigus vulgaris and 6 11 pemphigus foliaceus) versus an overall positive result of 47% for direct immunofluorescence ( 3 5 lupus erythematosus, 2 2 pemphigus vulgaris and 2 10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.

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