Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow

Rene Kronland, Thomas Grogan, Catherine Spier, Daniel Wirt, Catherine Rangel, Lynne Richter, Brian Durie, Bernard Greenberg, Thomas Miller, Stephen Jones

Research output: Contribution to journalArticle

  • 11 Citations

Abstract

To determine the utility of tissue section immunochemistry in the evaluation of bone marrow involved by lymphoid and plasma cell malignancies, snap-frozen, undecalcified bone marrow core and aspirate samples from 23 patients with these disorders were studied with a battery of monoclonal antibodies. With techniques that preserve architecture, difficult diagnostic cases characterized by core but not aspirate involvement, or the reverse, were resolved. By means of an extensive battery of monoclonal antibodies applied to serial sections, complex tumor cell phenotypes were established in all 23 cases. In addition to the identification of straghtfoward monoclonal surface immunoglobulin expression in small cleaved cell lymphomas (four cases), the battery approach added immunologic certainty in malignancies with unusual or difficult phenotypes: peripheral T-cell lymphomas with idiosyncratic antigen expression, and chronic lymphocitic leukemias and small cell lymphomas with faint surface immunoglobulin expression (four cases). For the chronic lymphocytic leukemias and the small cell lymphomas, the combined IgD+, B2+, B1+, Ia+, Leu-I+ phenotype taken as a whole had greater utility than any isolated marker. The acute lymphocytic leukemias and the myelomas studied demonstrate the wide range of B-cell antigens that must be detected to account for the variety of B-cell neoplasms encountered. Additionally, the previously undescribed phenotypic subset of CALLA+ myelomas, which is of prognostic relevance, was indentified. Marrow frozen section immunotyping is a major asset in the evaluation of patients with lymphoma, leukemia, and myeloma when special care is accorded to tissue handling and to treatment of endogenous peroxidase/pseudoperoxidase and interstitial immunoglobulin. © 1985 W. B. Saunders Co.

Original languageEnglish (US)
Pages (from-to)1247-1254
Number of pages8
JournalHuman Pathology
Volume16
Issue number12
DOIs
StatePublished - 1985
Externally publishedYes

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B-Cell Chronic Lymphocytic Leukemia
Plasma Cells
Bone Marrow
Lymphocytes
Phenotype
B-Cell Antigen Receptors
Leukemia
B-Lymphocytes
Monoclonal Antibodies
Antigens
Neoplasms
Peripheral T-Cell Lymphoma
Immunochemistry
Immunoglobulin D
Frozen Sections
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Peroxidase
Immunoglobulins
Lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Kronland, R., Grogan, T., Spier, C., Wirt, D., Rangel, C., Richter, L., ... Jones, S. (1985). Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow. Human Pathology, 16(12), 1247-1254. DOI: 10.1016/S0046-8177(85)80038-1

Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow. / Kronland, Rene; Grogan, Thomas; Spier, Catherine; Wirt, Daniel; Rangel, Catherine; Richter, Lynne; Durie, Brian; Greenberg, Bernard; Miller, Thomas; Jones, Stephen.

In: Human Pathology, Vol. 16, No. 12, 1985, p. 1247-1254.

Research output: Contribution to journalArticle

Kronland, R, Grogan, T, Spier, C, Wirt, D, Rangel, C, Richter, L, Durie, B, Greenberg, B, Miller, T & Jones, S 1985, 'Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow' Human Pathology, vol 16, no. 12, pp. 1247-1254. DOI: 10.1016/S0046-8177(85)80038-1
Kronland R, Grogan T, Spier C, Wirt D, Rangel C, Richter L et al. Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow. Human Pathology. 1985;16(12):1247-1254. Available from, DOI: 10.1016/S0046-8177(85)80038-1

Kronland, Rene; Grogan, Thomas; Spier, Catherine; Wirt, Daniel; Rangel, Catherine; Richter, Lynne; Durie, Brian; Greenberg, Bernard; Miller, Thomas; Jones, Stephen / Immunotopographic assessment of lymphoid and plasma cell malignancies in the bone marrow.

In: Human Pathology, Vol. 16, No. 12, 1985, p. 1247-1254.

Research output: Contribution to journalArticle

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