Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice

M. M. Hurley, Y. Okada, L. Xiao, Y. Tanaka, M. Ito, N. Okimoto, T. Nakamura, C. J. Rosen, Thomas C Doetschman, J. D. Coffin

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Since parathyroid hormone (PTH) increased FGF2 mRNA and protein expression in osteoblasts, and serum FGF-2 was increased in osteoporotic patients treated with PTH, we assessed whether the anabolic effect of PTH was impaired in Fgf2-/- mice. Eight-week-old Fgf2+/+ and Fgf2-/- male mice were treated with rhPTH 1-34 (80 μg/kg) for 4 weeks. Micro-CT and histomorphometry demonstrated that PTH significantly increased parameters of bone formation in femurs from Fgf2+/+ mice but the changes were smaller and not significant in Fgf2-/- mice. IGF-1 was significantly reduced in serum from PTH-treated Fgf2-/- mice. DEXA analysis of femurs from Fgf2+/+, Fgf2+/-, and Fgf2-/- mice treated with rhPTH (160 μg/kg) for 10 days showed that PTH significantly increased femoral BMD in Fgf2+/+ by 18%; by only 3% in Fgf2+/- mice and reduced by 3% in Fgf2-/- mice. We conclude that endogenous Fgf2 is important for maximum bone anabolic effect of PTH in mice.

Original languageEnglish (US)
Pages (from-to)989-994
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume341
Issue number4
DOIs
Publication statusPublished - Mar 24 2006
Externally publishedYes

    Fingerprint

Keywords

  • Bone formation
  • DEXA
  • Fgf2 haploinsufficient
  • Fgf2 null
  • Mice
  • Micro-CT
  • PTH

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hurley, M. M., Okada, Y., Xiao, L., Tanaka, Y., Ito, M., Okimoto, N., ... Coffin, J. D. (2006). Impaired bone anabolic response to parathyroid hormone in Fgf2-/- and Fgf2+/- mice. Biochemical and Biophysical Research Communications, 341(4), 989-994. https://doi.org/10.1016/j.bbrc.2006.01.044