Improved 2D inverse proton detected C,H correlation NMR techniques for the total assignment of carbon resonances of a highly delta opioid receptor agonist peptide

Katalin E. Kövér, Om Prakash, Victor J Hruby

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Assignment of the 13C resonances of the highly delta opioid receptor selective cyclic peptide (Formula Presented.) enkephalin (DPDPE) was accomplished by using single‐quantum inverse proton detected 2D heteronuclear correlation spectroscopy. The pulse sequences described provided better sensitivity and resolution than the corresponding, commonly used, multiple‐quantum evolution experiments, since during the SQ‐evolution period there is no line broadening due to scalar and dipolar 1H‐1H interactions. The proton detected C,H correlation spectrum, in combination with the H‐TOCSY relay C,H correlation, gave the assignment of all protonated carbons. The C,H correlation optimized for long‐range coupling gave the assignments of all non‐protonated carbons, such as the carbonyl carbons of the peptide bonds. These spectra also allowed the sequential assignment of this molecule.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalMagnetic Resonance in Chemistry
Volume31
Issue number3
DOIs
StatePublished - 1993

Keywords

  • Delta opioid receptor agonist peptide
  • Inverse experiments
  • Long‐range H,C correlations

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)

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