Improved data acquisition system for digital flow cytometry

Shiva Murthi, Sundararajan Sankaranarayanan, Bo Xia, Jeffrey J Rodriguez, David W Galbraith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Digital flow cytometry offers the flexibility to explore novel feature extraction and classification schemes for efficient sorting of biological cells [1,2]. A prototype of a second-generation digital data acquisition system (DDAPS-2) - a mixed-signal design operating at 40 MHz - was built to interface to a commercial flow cytometer. The DDAPS-2 intercepts the analog signal from the photomultiplier tube and preamp, performs analog-to-digital conversion, extracts various features and then feeds these extracted features into one of the several pattern classification algorithms. This paper describes the design and operation of the various sub-systems that constitute the DDAPS-2. The novelty of the DDAPS-2 is the use of dual-buffering FIFO memories to acquire digital samples of the pulse voltage signal. Experimental results demonstrate the improvement in the pulse capture performance of DDAPS-2 over DDAPS-1, which used a single-buffering FIFO memory.

Original languageEnglish (US)
Title of host publicationProceedings - IEEE International Symposium on Circuits and Systems
Volume1
StatePublished - 2002
Event2002 IEEE International Symposium on Circuits and Systems - Phoenix, AZ, United States
Duration: May 26 2002May 29 2002

Other

Other2002 IEEE International Symposium on Circuits and Systems
CountryUnited States
CityPhoenix, AZ
Period5/26/025/29/02

Fingerprint

Flow cytometry
Data acquisition
Data storage equipment
Photomultipliers
Analog to digital conversion
Sorting
Pattern recognition
Feature extraction
Electric potential

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

Cite this

Murthi, S., Sankaranarayanan, S., Xia, B., Rodriguez, J. J., & Galbraith, D. W. (2002). Improved data acquisition system for digital flow cytometry. In Proceedings - IEEE International Symposium on Circuits and Systems (Vol. 1)

Improved data acquisition system for digital flow cytometry. / Murthi, Shiva; Sankaranarayanan, Sundararajan; Xia, Bo; Rodriguez, Jeffrey J; Galbraith, David W.

Proceedings - IEEE International Symposium on Circuits and Systems. Vol. 1 2002.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Murthi, S, Sankaranarayanan, S, Xia, B, Rodriguez, JJ & Galbraith, DW 2002, Improved data acquisition system for digital flow cytometry. in Proceedings - IEEE International Symposium on Circuits and Systems. vol. 1, 2002 IEEE International Symposium on Circuits and Systems, Phoenix, AZ, United States, 5/26/02.
Murthi S, Sankaranarayanan S, Xia B, Rodriguez JJ, Galbraith DW. Improved data acquisition system for digital flow cytometry. In Proceedings - IEEE International Symposium on Circuits and Systems. Vol. 1. 2002
Murthi, Shiva ; Sankaranarayanan, Sundararajan ; Xia, Bo ; Rodriguez, Jeffrey J ; Galbraith, David W. / Improved data acquisition system for digital flow cytometry. Proceedings - IEEE International Symposium on Circuits and Systems. Vol. 1 2002.
@inproceedings{6a7cacd9172f41e68cfebf2919cd7ed5,
title = "Improved data acquisition system for digital flow cytometry",
abstract = "Digital flow cytometry offers the flexibility to explore novel feature extraction and classification schemes for efficient sorting of biological cells [1,2]. A prototype of a second-generation digital data acquisition system (DDAPS-2) - a mixed-signal design operating at 40 MHz - was built to interface to a commercial flow cytometer. The DDAPS-2 intercepts the analog signal from the photomultiplier tube and preamp, performs analog-to-digital conversion, extracts various features and then feeds these extracted features into one of the several pattern classification algorithms. This paper describes the design and operation of the various sub-systems that constitute the DDAPS-2. The novelty of the DDAPS-2 is the use of dual-buffering FIFO memories to acquire digital samples of the pulse voltage signal. Experimental results demonstrate the improvement in the pulse capture performance of DDAPS-2 over DDAPS-1, which used a single-buffering FIFO memory.",
author = "Shiva Murthi and Sundararajan Sankaranarayanan and Bo Xia and Rodriguez, {Jeffrey J} and Galbraith, {David W}",
year = "2002",
language = "English (US)",
volume = "1",
booktitle = "Proceedings - IEEE International Symposium on Circuits and Systems",

}

TY - GEN

T1 - Improved data acquisition system for digital flow cytometry

AU - Murthi, Shiva

AU - Sankaranarayanan, Sundararajan

AU - Xia, Bo

AU - Rodriguez, Jeffrey J

AU - Galbraith, David W

PY - 2002

Y1 - 2002

N2 - Digital flow cytometry offers the flexibility to explore novel feature extraction and classification schemes for efficient sorting of biological cells [1,2]. A prototype of a second-generation digital data acquisition system (DDAPS-2) - a mixed-signal design operating at 40 MHz - was built to interface to a commercial flow cytometer. The DDAPS-2 intercepts the analog signal from the photomultiplier tube and preamp, performs analog-to-digital conversion, extracts various features and then feeds these extracted features into one of the several pattern classification algorithms. This paper describes the design and operation of the various sub-systems that constitute the DDAPS-2. The novelty of the DDAPS-2 is the use of dual-buffering FIFO memories to acquire digital samples of the pulse voltage signal. Experimental results demonstrate the improvement in the pulse capture performance of DDAPS-2 over DDAPS-1, which used a single-buffering FIFO memory.

AB - Digital flow cytometry offers the flexibility to explore novel feature extraction and classification schemes for efficient sorting of biological cells [1,2]. A prototype of a second-generation digital data acquisition system (DDAPS-2) - a mixed-signal design operating at 40 MHz - was built to interface to a commercial flow cytometer. The DDAPS-2 intercepts the analog signal from the photomultiplier tube and preamp, performs analog-to-digital conversion, extracts various features and then feeds these extracted features into one of the several pattern classification algorithms. This paper describes the design and operation of the various sub-systems that constitute the DDAPS-2. The novelty of the DDAPS-2 is the use of dual-buffering FIFO memories to acquire digital samples of the pulse voltage signal. Experimental results demonstrate the improvement in the pulse capture performance of DDAPS-2 over DDAPS-1, which used a single-buffering FIFO memory.

UR - http://www.scopus.com/inward/record.url?scp=0036290371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036290371&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0036290371

VL - 1

BT - Proceedings - IEEE International Symposium on Circuits and Systems

ER -