Improved genome assembly of American alligator genome reveals conserved architecture of estrogen signaling

Edward S. Rice, Satomi Kohno, John St John, Son Pham, Jonathan Howard, Liana F. Lareau, Brendan L. O'Connell, Glenn Hickey, Joel Armstrong, Alden Deran, Ian Fiddes, Roy N. Platt, Cathy Gresham, Fiona M McCarthy, Colin Kern, David Haan, Tan Phan, Carl Schmidt, Jeremy R. Sanford, David A. Ray & 3 others Benedict Paten, Louis J. Guillette, Richard E. Green

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The American alligator, Alligator mississippiensis, like all crocodilians, has temperature-dependent sex determination, in which the sex of an embryo is determined by the incubation temperature of the egg during a critical period of development. The lack of genetic differences between male and female alligators leaves open the question of how the genes responsible for sex determination and differentiation are regulated. Insight into this question comes from the fact that exposing an embryo incubated at male-producing temperature to estrogen causes it to develop ovaries. Because estrogen response elements are known to regulate genes over long distances, a contiguous genome assembly is crucial for predicting and understanding their impact. We present an improved assembly of the American alligator genome, scaffolded with in vitro proximity ligation (Chicago) data. We use this assembly to scaffold two other crocodilian genomes based on synteny. We perform RNA sequencing of tissues from American alligator embryos to find genes that are differentially expressed between embryos incubated at male- versus female-producing temperature. Finally, we use the improved contiguity of our assembly along with the current model of CTCF-mediated chromatin looping to predict regions of the genome likely to contain estrogen-responsive genes. We find that these regions are significantly enriched for genes with female-biased expression in developing gonads after the critical period during which sex is determined by incubation temperature. We thus conclude that estrogen signaling is a major driver of female-biased gene expression in the posterature sensitive period gonads.

Original languageEnglish (US)
Pages (from-to)686-696
Number of pages11
JournalGenome Research
Volume27
Issue number5
DOIs
StatePublished - May 1 2017

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Alligators and Crocodiles
Estrogens
Genome
Embryonic Structures
Temperature
Genes
Gonads
RNA Sequence Analysis
Synteny
Safe Sex
Sex Differentiation
Response Elements
Chromatin
Ovum
Ligation
Ovary
Gene Expression

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Rice, E. S., Kohno, S., St John, J., Pham, S., Howard, J., Lareau, L. F., ... Green, R. E. (2017). Improved genome assembly of American alligator genome reveals conserved architecture of estrogen signaling. Genome Research, 27(5), 686-696. https://doi.org/10.1101/gr.213595.116

Improved genome assembly of American alligator genome reveals conserved architecture of estrogen signaling. / Rice, Edward S.; Kohno, Satomi; St John, John; Pham, Son; Howard, Jonathan; Lareau, Liana F.; O'Connell, Brendan L.; Hickey, Glenn; Armstrong, Joel; Deran, Alden; Fiddes, Ian; Platt, Roy N.; Gresham, Cathy; McCarthy, Fiona M; Kern, Colin; Haan, David; Phan, Tan; Schmidt, Carl; Sanford, Jeremy R.; Ray, David A.; Paten, Benedict; Guillette, Louis J.; Green, Richard E.

In: Genome Research, Vol. 27, No. 5, 01.05.2017, p. 686-696.

Research output: Contribution to journalArticle

Rice, ES, Kohno, S, St John, J, Pham, S, Howard, J, Lareau, LF, O'Connell, BL, Hickey, G, Armstrong, J, Deran, A, Fiddes, I, Platt, RN, Gresham, C, McCarthy, FM, Kern, C, Haan, D, Phan, T, Schmidt, C, Sanford, JR, Ray, DA, Paten, B, Guillette, LJ & Green, RE 2017, 'Improved genome assembly of American alligator genome reveals conserved architecture of estrogen signaling', Genome Research, vol. 27, no. 5, pp. 686-696. https://doi.org/10.1101/gr.213595.116
Rice, Edward S. ; Kohno, Satomi ; St John, John ; Pham, Son ; Howard, Jonathan ; Lareau, Liana F. ; O'Connell, Brendan L. ; Hickey, Glenn ; Armstrong, Joel ; Deran, Alden ; Fiddes, Ian ; Platt, Roy N. ; Gresham, Cathy ; McCarthy, Fiona M ; Kern, Colin ; Haan, David ; Phan, Tan ; Schmidt, Carl ; Sanford, Jeremy R. ; Ray, David A. ; Paten, Benedict ; Guillette, Louis J. ; Green, Richard E. / Improved genome assembly of American alligator genome reveals conserved architecture of estrogen signaling. In: Genome Research. 2017 ; Vol. 27, No. 5. pp. 686-696.
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