Improved MALDI-TOF Imaging Yields Increased Protein Signals at High Molecular Mass

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Abstract

Matrix assisted laser desorption ionization (MALDI) mass spectrum images are created from an array of mass spectra collected over a tissue surface. We have increased the mass range of proteins that can be detected in tissue sections from kidneys, heart, lung and brain of different rodent species by a modification of the sandwich technique, which involves co-crystallizing matrix with analyte. A tissue section is placed upon a drop of sinapinic acid matrix dissolved in 90% ethanol and 0.5% Triton X-100. Once the matrix has dried, a seed layer of sinapinic crystals is added as a dispersion in xylene. Additional layers of sinapinic acid are added as solutions in 90% ethanol followed by 50% acetonitrile. Numerous peaks with signal to noise ratio of four or greater are observed between 25 kDa to 50 kDa. This represents ∼10 times as many peaks as are detected using traditional matrix spotting and spraying.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Volume20
Issue number1
DOIs
StatePublished - Jan 2009

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Molecular mass
Ionization
Desorption
Lasers
Tissue
Imaging techniques
Ethanol
Xylenes
Metrorrhagia
Proteins
Octoxynol
Signal-To-Noise Ratio
Spraying
Seed
Rodentia
Brain
Seeds
Signal to noise ratio
Kidney
Lung

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

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abstract = "Matrix assisted laser desorption ionization (MALDI) mass spectrum images are created from an array of mass spectra collected over a tissue surface. We have increased the mass range of proteins that can be detected in tissue sections from kidneys, heart, lung and brain of different rodent species by a modification of the sandwich technique, which involves co-crystallizing matrix with analyte. A tissue section is placed upon a drop of sinapinic acid matrix dissolved in 90{\%} ethanol and 0.5{\%} Triton X-100. Once the matrix has dried, a seed layer of sinapinic crystals is added as a dispersion in xylene. Additional layers of sinapinic acid are added as solutions in 90{\%} ethanol followed by 50{\%} acetonitrile. Numerous peaks with signal to noise ratio of four or greater are observed between 25 kDa to 50 kDa. This represents ∼10 times as many peaks as are detected using traditional matrix spotting and spraying.",
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