Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Lisa F. Shubitz, Jieh Juen Yu, Chiung Yu Hung, Theo N. Kirkland, Tao Peng, Robert Perrill, Julie Simons, Jianmin Xue, Roger A. Herr, Garry T. Cole, John N. Galgiani

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

Original languageEnglish (US)
Pages (from-to)5904-5911
Number of pages8
JournalVaccine
Volume24
Issue number31-32
DOIs
StatePublished - Jul 26 2006

Keywords

  • Cell-mediated immunity
  • Coccidioides
  • Mouse
  • Recombinant expression protein
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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