Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

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16 Scopus citations

Abstract

Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalDiseases of aquatic organisms
Volume80
Issue number1
DOIs
StatePublished - Jun 19 2008

Keywords

  • NHP
  • Necrotizing hepatopancreatitis
  • PCR
  • Penaeus vannamei
  • Shrimp

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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