Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalDiseases of Aquatic Organisms
Volume80
Issue number1
DOIs
StatePublished - Jun 19 2008

Fingerprint

necrotizing hepatopancreatitis
shrimp
microbial detection
shrimp culture
bacterial disease
Penaeidae
alpha-Proteobacteria
pathology
aquaculture
pathogen
DNA
bacterium
bacteria
methodology
detection
method

Keywords

  • Necrotizing hepatopancreatitis
  • NHP
  • PCR
  • Penaeus vannamei
  • Shrimp

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp. / Nunan, Linda M.; Pantoja-Morales, Carlos R; Lightner, Donald V.

In: Diseases of Aquatic Organisms, Vol. 80, No. 1, 19.06.2008, p. 69-73.

Research output: Contribution to journalArticle

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