In-cell RT-PCR in a single, detached plant cell

Ho Hyung Woo, Lindy A. Brigham, Martha C Hawes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.

Original languageEnglish (US)
Pages (from-to)355-362
Number of pages8
JournalPlant Molecular Biology Reporter
Volume13
Issue number4
DOIs
StatePublished - Dec 1995

Fingerprint

Digoxigenin
Polymerase chain reaction
Plant Cells
Transcription
Aldehydes
Reverse Transcription
Alkaline Phosphatase
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
digoxigenin
Messenger RNA
Peas
Seedlings
aldehydes
alkaline phosphatase
peas
cells
seedlings

Keywords

  • digoxigenin-dUTP
  • histone H1
  • In-cell RT-PCR
  • single detached plant cell

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

In-cell RT-PCR in a single, detached plant cell. / Woo, Ho Hyung; Brigham, Lindy A.; Hawes, Martha C.

In: Plant Molecular Biology Reporter, Vol. 13, No. 4, 12.1995, p. 355-362.

Research output: Contribution to journalArticle

Woo, Ho Hyung ; Brigham, Lindy A. ; Hawes, Martha C. / In-cell RT-PCR in a single, detached plant cell. In: Plant Molecular Biology Reporter. 1995 ; Vol. 13, No. 4. pp. 355-362.
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