In-cell RT-PCR in a single, detached plant cell

Ho Hyung Woo, Lindy A. Brigham, Martha C. Hawes

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.

Original languageEnglish (US)
Pages (from-to)355-362
Number of pages8
JournalPlant Molecular Biology Reporter
Volume13
Issue number4
DOIs
StatePublished - Dec 1 1995

Keywords

  • In-cell RT-PCR
  • digoxigenin-dUTP
  • histone H1
  • single detached plant cell

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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