In search of susceptibility genes for type 2 diabetes in West Africa: The design and results of the first phase of the AADM study

Charles N. Rotimi, Georgia M. Dunston, Kate Berg, Omobosola Akinsete, Albert Amoah, Samuel Owusu, Joseph Acheampong, Kofi Boateng, Johnnie Oli, Godfrey Okafor, Belonwu Onyenekwe, Babatunde Osotimehin, Fayeofori Abbiyesuku, Thomas Johnson, Olufemi Fasanmade, Paulette Furbert-Harris, Rick Kittles, Mirjana Vekich, Olufemi Adegoke, George BonneyFrancis Collins

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

PURPOSE: The purpose of this study is to map type 2 diabetes susceptibility genes in West African ancestral populations of African-Americans, through an international collaboration between West African and US investigators. DESIGN AND METHODS: Affected sib-pairs (ASP) along with unaffected spouse controls are being enrolled and examined in West Africa, with two sites established in Ghana (Accra and Kumasi) and three in Nigeria (Enugu, Ibadan, and Lagos). Eligible participants are invited to study clinics to obtain detailed epidemiologic, family, and medical history information. Blood samples are drawn from each participant to measure glucose, insulin, C-peptide, total cholesterol, LDL, HDL, triglycerides, albumin, creatinine, urea, uric acid, total calcium and to detect autoantibodies to glutamic acid decarboxylase (GAD). DNA is isolated from frozen white blood cells obtained from 20 ml of EDTA whole blood samples. RESULTS: With full informed consent, 162 individuals from 78 families have been enrolled and examined since the Africa America Diabetes Mellitus (AADM) study began in June of 1997. Logistics of field examinations and specimen shipping have been successfully established. At the end of the third year of field activity (September 2000) the AADM study will have enrolled and performed comprehensive examination on 400 ASP with type 2 diabetes, for a minimum of 800 cases and 200 controls from Ghana and Nigeria. At the current participation rate, the goal of 400 sib-pairs and 200 controls will be met before the scheduled closing date. CONCLUSIONS: The AADM study will create a comprehensive epidemiologic and genetic resource that will facilitate a powerful genome-wide search for West African susceptibility genes to type 2 diabetes.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalAnnals of epidemiology
Volume11
Issue number1
DOIs
StatePublished - Jan 30 2001
Externally publishedYes

Keywords

  • Genes
  • Linkage
  • Sib-Pairs
  • Type 2 diabetes
  • West Africa

ASJC Scopus subject areas

  • Epidemiology

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    Rotimi, C. N., Dunston, G. M., Berg, K., Akinsete, O., Amoah, A., Owusu, S., Acheampong, J., Boateng, K., Oli, J., Okafor, G., Onyenekwe, B., Osotimehin, B., Abbiyesuku, F., Johnson, T., Fasanmade, O., Furbert-Harris, P., Kittles, R., Vekich, M., Adegoke, O., ... Collins, F. (2001). In search of susceptibility genes for type 2 diabetes in West Africa: The design and results of the first phase of the AADM study. Annals of epidemiology, 11(1), 51-58. https://doi.org/10.1016/S1047-2797(00)00180-0