In situ analysis of microRNA expression during vertebrate development

Diana K. Darnell, Stacey Stanislaw, Simran Kaur, Tatiana A. Yatskievych, Sean Davey, Jay H. Konieczka, Parker B Antin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Introduction A widespread class of non-coding, regulatory RNAs has been recently characterized. Because of their short length (21-22 nucleotides) they are called microRNA or miRNA. These miRNAs have been identified in diverse organisms (prokaryote, eukaryote, vertebrates, invertebrates, plants, fungi) and in viruses (Tuschl et al., 1999; Elbashir et al., 2001; Griffiths-Jones, 2004; Berezikov and Plasterk, 2005; Griffiths-Jones et al., 2006). Their apparently ancient function is to regulate specific protein concentration by inhibiting the first step of translation or by inducing specific mRNA degradation by 3' UTR binding (He and Hannon, 2004; Pillai, 2005; Valencia-Sanchez et al., 2006). Both molecular and bioinformatics tools have been used to identify candidate miRNAs and their target mRNAs. Based on the numbers generated in these studies, it is estimated that vertebrate genomes may contain hundreds of miRNA genes that may regulate stability or translation of approximately one quarter of all mRNAs (Bentwich et al., 2005; Berezikov and Plasterk, 2005; Legendre et al., 2005; Xie et al., 2005). Disruption of miRNA function often produces aberrations of important processes including organogenesis, and cell diversification, proliferation, and survival (Reinhart et al., 2000; Brennecke et al., 2003; Dostie et al., 2003; Ambros, 2004; Calin et al., 2004; Alvarez-Garcia and Miska, 2005; Giraldez et al., 2005). The miRNA function has also been implicated in regulating stem cell renewal and the onset of certain cancers (Hatfield et al., 2005, Lu et al., 2005). Therefore, miRNAs regulate important processes in animal development, physiology and disease.

Original languageEnglish (US)
Title of host publicationMicroRNAs: From Basic Science to Disease Biology
PublisherCambridge University Press
Pages102-114
Number of pages13
ISBN (Print)9780511541766, 9780521865982
DOIs
StatePublished - Jan 1 2007

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MicroRNAs
Vertebrates
Messenger RNA
Genes
Untranslated RNA
Organogenesis
RNA Stability
Physiology
Cell proliferation
3' Untranslated Regions
Invertebrates
Bioinformatics
Computational Biology
Stem cells
Eukaryota
Fungi
Aberrations
Viruses
Cell Survival
Animals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Darnell, D. K., Stanislaw, S., Kaur, S., Yatskievych, T. A., Davey, S., Konieczka, J. H., & Antin, P. B. (2007). In situ analysis of microRNA expression during vertebrate development. In MicroRNAs: From Basic Science to Disease Biology (pp. 102-114). Cambridge University Press. https://doi.org/10.1017/CBO9780511541766.010

In situ analysis of microRNA expression during vertebrate development. / Darnell, Diana K.; Stanislaw, Stacey; Kaur, Simran; Yatskievych, Tatiana A.; Davey, Sean; Konieczka, Jay H.; Antin, Parker B.

MicroRNAs: From Basic Science to Disease Biology. Cambridge University Press, 2007. p. 102-114.

Research output: Chapter in Book/Report/Conference proceedingChapter

Darnell, DK, Stanislaw, S, Kaur, S, Yatskievych, TA, Davey, S, Konieczka, JH & Antin, PB 2007, In situ analysis of microRNA expression during vertebrate development. in MicroRNAs: From Basic Science to Disease Biology. Cambridge University Press, pp. 102-114. https://doi.org/10.1017/CBO9780511541766.010
Darnell DK, Stanislaw S, Kaur S, Yatskievych TA, Davey S, Konieczka JH et al. In situ analysis of microRNA expression during vertebrate development. In MicroRNAs: From Basic Science to Disease Biology. Cambridge University Press. 2007. p. 102-114 https://doi.org/10.1017/CBO9780511541766.010
Darnell, Diana K. ; Stanislaw, Stacey ; Kaur, Simran ; Yatskievych, Tatiana A. ; Davey, Sean ; Konieczka, Jay H. ; Antin, Parker B. / In situ analysis of microRNA expression during vertebrate development. MicroRNAs: From Basic Science to Disease Biology. Cambridge University Press, 2007. pp. 102-114
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