In situ detection of human β1-interferon mRNA with a chemically biotinylated probe

Feng-Jyu Tang-Nelson, Nobuko Kakiuchi, Paul O.P. Ts'o, Stephen A. Lesko

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This paper describes a rapid, nonradioactive in situ mRNA hybridization method through the use of a chemically biotinylated DNA probe for the detection of human β1-IFN mRNA. The chemically biotinylated DNA probe is characterized with respects to: (1) the percentage of modification in nucleic acids analyzed by HPLC, (2) the sensitivity of detection, determined by reacting a series of quantities of biotinylated DNA with streptavidin-alkaline phosphatase, and (3) the hybridization specificity determined by hybridizing the biotinylated DNA to the in vitro synthesized β1-IFN sense RNA dotted on the filter. The optimal conditions of in situ mRNA hybridization were determined by investigating its specificity to the β1-IFN mRNA in the uninduced, or RNase-treated induced, or induced cells.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalActa Histochemica et Cytochemica
Volume26
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

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