In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing

Mandy L.Y. Sin, Tingting Liu, Jeffrey D. Pyne, Vincent Gau, Joseph C. Liao, Pak Kin Wong

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

This study reports a multifunctional electrode approach which directly implements electrokinetic enhancement on a self-assembled-monolayer-based electrochemical sensor for point-of-care diagnostics. Using urinary tract infections as a model system, we demonstrate that electrokinetic enhancement, which involves in situ stirring and heating, can enhance the sensitivity of the strain specific 16S rRNA hybridization assay for 1 order of magnitude and accelerate the time-limiting incubation step with a 6-fold reduction in the incubation time. Since the same electrode platform is used for both electrochemical signal enhancement and electrochemical sensing, the multifunctional electrode approach provides a highly effective strategy toward fully integrated lab-on-a-chip systems for various biomedical applications.

Original languageEnglish (US)
Pages (from-to)2702-2707
Number of pages6
JournalAnalytical chemistry
Volume84
Issue number6
DOIs
StatePublished - Mar 20 2012

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ASJC Scopus subject areas

  • Analytical Chemistry

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