In vitro age-related responses of endothelial cells to breast cancer cell addition

Carrie J Merkle, Bonny J. Torres, Jean M. Baruch, Katherine Stevens, Christine Munoz, Richard C. Schaeffer, David W Montgomery

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: The purpose of this study was to determine if the in vitro age of endothelial cells alters endothelial response(s) to breast cancer cells. Method: After characterizing lower passage ("young"; passages 10-16) and higher passage ("old"; passages 30-36) bovine pulmonary artery endothelial cells (BPAECs), fluorescently labeled MCF-7 breast cancer cells were added to confluent monolayers of young and old BPAECs. Results: Transient gaps that peaked in size by 12 h and closed by 48 h occurred between the young BPAECs, while large persistent gaps formed between the old BPAECs. Gap formation did not occur when 184A1 cells, a non-malignant mammary epithelial cell line, were added in place of MCF-7 cells, suggesting that the age-related responses of the endothelial cells to MCF-7 cell addition were specific to the tumor cell addition. Additionally, more MCF-7 cells migrated through old BPAEC monolayers, than young BPAEC monolayers, grown on Matrigel™-coated filters. Finally, DNA fragmentation and fluorescent annexin-V binding assays suggested increased MCF-7 cell-induced apoptosis in older BPAECs, though results from a caspase-3 activation assay were equivocal. Conclusions: In sum, our findings support the notion that aged endothelial cells are more susceptible to breast cancer-induced injury, perhaps due to increased apoptosis.

Original languageEnglish (US)
Pages (from-to)518-527
Number of pages10
JournalCancer Detection and Prevention
Volume29
Issue number6
DOIs
StatePublished - 2005

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Endothelial Cells
Breast Neoplasms
Pulmonary Artery
MCF-7 Cells
In Vitro Techniques
Apoptosis
Annexin A5
DNA Fragmentation
Caspase 3
Breast
Epithelial Cells
Cell Line
Wounds and Injuries
Neoplasms

Keywords

  • Apoptosis
  • Bovine pulmonary artery endothelial cells (BPAEC)
  • Breast cancer cells
  • Co-culture experiments
  • Endothelial aging model
  • Gap formation
  • In vitro response
  • MCF-7 cells
  • Metastasis
  • Microscopy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro age-related responses of endothelial cells to breast cancer cell addition. / Merkle, Carrie J; Torres, Bonny J.; Baruch, Jean M.; Stevens, Katherine; Munoz, Christine; Schaeffer, Richard C.; Montgomery, David W.

In: Cancer Detection and Prevention, Vol. 29, No. 6, 2005, p. 518-527.

Research output: Contribution to journalArticle

Merkle, Carrie J ; Torres, Bonny J. ; Baruch, Jean M. ; Stevens, Katherine ; Munoz, Christine ; Schaeffer, Richard C. ; Montgomery, David W. / In vitro age-related responses of endothelial cells to breast cancer cell addition. In: Cancer Detection and Prevention. 2005 ; Vol. 29, No. 6. pp. 518-527.
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