In Vitro Analyses of Interactions between Olfactory Receptor Growth Cones and Glial Cells That Mediate Axon Sorting and Glomerulus Formation

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

During development, the axons of olfactory receptor neurons project to the CNS and converge on glomerular targets. For vertebrate and invertebrate olfactory systems, neuron-glia interactions have been hypothesized to regulate the sorting and targeting of olfactory receptor axons and the development of glomeruli. In the moth Manduca sexta, glial reduction experiments have directly implicated two types of central olfactory glia, the sorting zone- and neuropil-associated glia, in key events in olfactory development, including axon sorting and glomerulus stabilization. By using cocultures containing central olfactory glial cells and explants of olfactory receptor epithelium, we show that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone- and neuropil-associated glial cells. These effects on growth cone behavior were specific to central olfactory glia; peripheral glial cells of the olfactory nerve failed to elicit similar responses in olfactory receptor growth cones. We propose that sorting zone- and neuropil-associated glial cells similarly modify axon behavior in vitro by altering the adhesive properties and cytoskeleton of olfactory receptor growth cones and that these in vitro changes may underlie functionally relevant changes in growth cone behavior in vivo.

Original languageEnglish (US)
Pages (from-to)478-495
Number of pages18
JournalJournal of Comparative Neurology
Volume472
Issue number4
DOIs
StatePublished - May 10 2004

Fingerprint

Odorant Receptors
Growth Cones
Neuroglia
Axons
Neuropil
Olfactory Receptor Neurons
Olfactory Nerve
Manduca
In Vitro Techniques
Olfactory Mucosa
Moths
Invertebrates
Coculture Techniques
Cytoskeleton
Adhesives
Vertebrates

Keywords

  • Development
  • Manduca sexta
  • Neuron-glia interaction
  • Olfaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{f716b4957baa4099b0024c1e408a76d4,
title = "In Vitro Analyses of Interactions between Olfactory Receptor Growth Cones and Glial Cells That Mediate Axon Sorting and Glomerulus Formation",
abstract = "During development, the axons of olfactory receptor neurons project to the CNS and converge on glomerular targets. For vertebrate and invertebrate olfactory systems, neuron-glia interactions have been hypothesized to regulate the sorting and targeting of olfactory receptor axons and the development of glomeruli. In the moth Manduca sexta, glial reduction experiments have directly implicated two types of central olfactory glia, the sorting zone- and neuropil-associated glia, in key events in olfactory development, including axon sorting and glomerulus stabilization. By using cocultures containing central olfactory glial cells and explants of olfactory receptor epithelium, we show that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone- and neuropil-associated glial cells. These effects on growth cone behavior were specific to central olfactory glia; peripheral glial cells of the olfactory nerve failed to elicit similar responses in olfactory receptor growth cones. We propose that sorting zone- and neuropil-associated glial cells similarly modify axon behavior in vitro by altering the adhesive properties and cytoskeleton of olfactory receptor growth cones and that these in vitro changes may underlie functionally relevant changes in growth cone behavior in vivo.",
keywords = "Development, Manduca sexta, Neuron-glia interaction, Olfaction",
author = "Tucker, {Eric S.} and Oland, {Lynne A} and Tolbert, {Leslie P}",
year = "2004",
month = "5",
day = "10",
doi = "10.1002/cne.20058",
language = "English (US)",
volume = "472",
pages = "478--495",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - In Vitro Analyses of Interactions between Olfactory Receptor Growth Cones and Glial Cells That Mediate Axon Sorting and Glomerulus Formation

AU - Tucker, Eric S.

AU - Oland, Lynne A

AU - Tolbert, Leslie P

PY - 2004/5/10

Y1 - 2004/5/10

N2 - During development, the axons of olfactory receptor neurons project to the CNS and converge on glomerular targets. For vertebrate and invertebrate olfactory systems, neuron-glia interactions have been hypothesized to regulate the sorting and targeting of olfactory receptor axons and the development of glomeruli. In the moth Manduca sexta, glial reduction experiments have directly implicated two types of central olfactory glia, the sorting zone- and neuropil-associated glia, in key events in olfactory development, including axon sorting and glomerulus stabilization. By using cocultures containing central olfactory glial cells and explants of olfactory receptor epithelium, we show that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone- and neuropil-associated glial cells. These effects on growth cone behavior were specific to central olfactory glia; peripheral glial cells of the olfactory nerve failed to elicit similar responses in olfactory receptor growth cones. We propose that sorting zone- and neuropil-associated glial cells similarly modify axon behavior in vitro by altering the adhesive properties and cytoskeleton of olfactory receptor growth cones and that these in vitro changes may underlie functionally relevant changes in growth cone behavior in vivo.

AB - During development, the axons of olfactory receptor neurons project to the CNS and converge on glomerular targets. For vertebrate and invertebrate olfactory systems, neuron-glia interactions have been hypothesized to regulate the sorting and targeting of olfactory receptor axons and the development of glomeruli. In the moth Manduca sexta, glial reduction experiments have directly implicated two types of central olfactory glia, the sorting zone- and neuropil-associated glia, in key events in olfactory development, including axon sorting and glomerulus stabilization. By using cocultures containing central olfactory glial cells and explants of olfactory receptor epithelium, we show that olfactory receptor growth cones elaborate extensively and cease advancement following contact with sorting zone- and neuropil-associated glial cells. These effects on growth cone behavior were specific to central olfactory glia; peripheral glial cells of the olfactory nerve failed to elicit similar responses in olfactory receptor growth cones. We propose that sorting zone- and neuropil-associated glial cells similarly modify axon behavior in vitro by altering the adhesive properties and cytoskeleton of olfactory receptor growth cones and that these in vitro changes may underlie functionally relevant changes in growth cone behavior in vivo.

KW - Development

KW - Manduca sexta

KW - Neuron-glia interaction

KW - Olfaction

UR - http://www.scopus.com/inward/record.url?scp=1942420796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942420796&partnerID=8YFLogxK

U2 - 10.1002/cne.20058

DO - 10.1002/cne.20058

M3 - Article

C2 - 15065121

AN - SCOPUS:1942420796

VL - 472

SP - 478

EP - 495

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 4

ER -