In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli

C. E. Krull, Lynne A Oland, A. Faissner, M. Schachner, Leslie P Tolbert

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Tenascin-like material is associated with glial cells that form borders around developing glomerular units in the olfactory (antennal) lobe of the moth Manduca sexta and is present at critical stages of glomerulus formation (Krull et al., 1994, J. Neurobiol. 25:515-534). Tenascin-like immunoreactivity declines in the mature lobe, coincident with a wave of synapse formation within the glomeruli and glomerulus stabilization. Tenascin-like molecules associated with neuropilar glia are in the correct position to influence the branching patterns of growing neurites by constraining them to glomeruli. In this study, we examine the growth of cultured moth antennal-lobe neurons in response to mouse CNS tenascin. Uniform tenascin provides a poor substrate for cell-body attachment and neurite outgrowth. Neuronal cell bodies provided with a striped substratum consisting of tenascin and concanavalin-A (con-A)/laminin attach preferentially to con-A/laminin lanes. Most neurons restrict their branching to con-A/laminin lanes both at early and later times in culture but others send processes across multiple tenascin and con-/laminin lanes in an apparently indiscriminate manner. Tenascin can inhibit the neuritic outgrowth of most antennal-lobe neurons, and this raises the possibility that the tenascin-like molecules associated with neuropilar glia in vivo act to constrain growing neurites to glomeruli. Thus, glial cells, acting in concert with olfactory axons, might act to promote glomerular patterns of branching by antennal-lobe neurons.

Original languageEnglish (US)
Pages (from-to)989-1004
Number of pages16
JournalJournal of Neurobiology
Volume25
Issue number8
DOIs
StatePublished - 1994

Fingerprint

Tenascin
Olfactory Bulb
Insects
Laminin
Neuroglia
Concanavalin A
Neurons
Moths
Neurites
Manduca
Neuronal Outgrowth
In Vitro Techniques
Synapses
Axons

Keywords

  • development
  • glia
  • glomeruli
  • olfaction
  • tenascin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli. / Krull, C. E.; Oland, Lynne A; Faissner, A.; Schachner, M.; Tolbert, Leslie P.

In: Journal of Neurobiology, Vol. 25, No. 8, 1994, p. 989-1004.

Research output: Contribution to journalArticle

@article{ad984be7ac304af2b29160517eaacfa2,
title = "In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli",
abstract = "Tenascin-like material is associated with glial cells that form borders around developing glomerular units in the olfactory (antennal) lobe of the moth Manduca sexta and is present at critical stages of glomerulus formation (Krull et al., 1994, J. Neurobiol. 25:515-534). Tenascin-like immunoreactivity declines in the mature lobe, coincident with a wave of synapse formation within the glomeruli and glomerulus stabilization. Tenascin-like molecules associated with neuropilar glia are in the correct position to influence the branching patterns of growing neurites by constraining them to glomeruli. In this study, we examine the growth of cultured moth antennal-lobe neurons in response to mouse CNS tenascin. Uniform tenascin provides a poor substrate for cell-body attachment and neurite outgrowth. Neuronal cell bodies provided with a striped substratum consisting of tenascin and concanavalin-A (con-A)/laminin attach preferentially to con-A/laminin lanes. Most neurons restrict their branching to con-A/laminin lanes both at early and later times in culture but others send processes across multiple tenascin and con-/laminin lanes in an apparently indiscriminate manner. Tenascin can inhibit the neuritic outgrowth of most antennal-lobe neurons, and this raises the possibility that the tenascin-like molecules associated with neuropilar glia in vivo act to constrain growing neurites to glomeruli. Thus, glial cells, acting in concert with olfactory axons, might act to promote glomerular patterns of branching by antennal-lobe neurons.",
keywords = "development, glia, glomeruli, olfaction, tenascin",
author = "Krull, {C. E.} and Oland, {Lynne A} and A. Faissner and M. Schachner and Tolbert, {Leslie P}",
year = "1994",
doi = "10.1002/neu.480250808",
language = "English (US)",
volume = "25",
pages = "989--1004",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "8",

}

TY - JOUR

T1 - In vitro analyses of neurite outgrowth indicate a potential role for tenascin-like molecules in the development of insect olfactory glomeruli

AU - Krull, C. E.

AU - Oland, Lynne A

AU - Faissner, A.

AU - Schachner, M.

AU - Tolbert, Leslie P

PY - 1994

Y1 - 1994

N2 - Tenascin-like material is associated with glial cells that form borders around developing glomerular units in the olfactory (antennal) lobe of the moth Manduca sexta and is present at critical stages of glomerulus formation (Krull et al., 1994, J. Neurobiol. 25:515-534). Tenascin-like immunoreactivity declines in the mature lobe, coincident with a wave of synapse formation within the glomeruli and glomerulus stabilization. Tenascin-like molecules associated with neuropilar glia are in the correct position to influence the branching patterns of growing neurites by constraining them to glomeruli. In this study, we examine the growth of cultured moth antennal-lobe neurons in response to mouse CNS tenascin. Uniform tenascin provides a poor substrate for cell-body attachment and neurite outgrowth. Neuronal cell bodies provided with a striped substratum consisting of tenascin and concanavalin-A (con-A)/laminin attach preferentially to con-A/laminin lanes. Most neurons restrict their branching to con-A/laminin lanes both at early and later times in culture but others send processes across multiple tenascin and con-/laminin lanes in an apparently indiscriminate manner. Tenascin can inhibit the neuritic outgrowth of most antennal-lobe neurons, and this raises the possibility that the tenascin-like molecules associated with neuropilar glia in vivo act to constrain growing neurites to glomeruli. Thus, glial cells, acting in concert with olfactory axons, might act to promote glomerular patterns of branching by antennal-lobe neurons.

AB - Tenascin-like material is associated with glial cells that form borders around developing glomerular units in the olfactory (antennal) lobe of the moth Manduca sexta and is present at critical stages of glomerulus formation (Krull et al., 1994, J. Neurobiol. 25:515-534). Tenascin-like immunoreactivity declines in the mature lobe, coincident with a wave of synapse formation within the glomeruli and glomerulus stabilization. Tenascin-like molecules associated with neuropilar glia are in the correct position to influence the branching patterns of growing neurites by constraining them to glomeruli. In this study, we examine the growth of cultured moth antennal-lobe neurons in response to mouse CNS tenascin. Uniform tenascin provides a poor substrate for cell-body attachment and neurite outgrowth. Neuronal cell bodies provided with a striped substratum consisting of tenascin and concanavalin-A (con-A)/laminin attach preferentially to con-A/laminin lanes. Most neurons restrict their branching to con-A/laminin lanes both at early and later times in culture but others send processes across multiple tenascin and con-/laminin lanes in an apparently indiscriminate manner. Tenascin can inhibit the neuritic outgrowth of most antennal-lobe neurons, and this raises the possibility that the tenascin-like molecules associated with neuropilar glia in vivo act to constrain growing neurites to glomeruli. Thus, glial cells, acting in concert with olfactory axons, might act to promote glomerular patterns of branching by antennal-lobe neurons.

KW - development

KW - glia

KW - glomeruli

KW - olfaction

KW - tenascin

UR - http://www.scopus.com/inward/record.url?scp=0028246691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028246691&partnerID=8YFLogxK

U2 - 10.1002/neu.480250808

DO - 10.1002/neu.480250808

M3 - Article

C2 - 7525872

AN - SCOPUS:0028246691

VL - 25

SP - 989

EP - 1004

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 8

ER -