In vitro assessment of macrophage attachment and phenotype on polymerized phospholipid bilayers

Jonathan Page, Benjamin A. Heitz, James R. Joubert, John P. Keogh, Tim Sparer, S. Scott Saavedra, Wei He

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Phosphatidyl choline (PC)-based materials have been found to be resistant to nonspecific protein adhesion in vitro. In this study, a PC-based planar supported phospholipid bilayer composed of 1,2-bis[10-(2′,4′- hexadienoyloxy)decanoyl]-sn-glycero-3-phosphocholine (bis-SorbPC or BSPC) was generated on piranha-treated silicon wafers by vesicle deposition. The bilayer was polymerized with redox initiation forming a stable 4-nm thick coating. Polymerized lipid bilayers (PLBs) were characterized and tested for uniformity, with ellipsometry and contact angle. Cellular adhesion and morphological changes in RAW 264.7 macrophages were investigated in vitro on PLBs and compared to bare silicon controls. Fluorescent and scanning electron microscopy were used to observe changes in cellular morphology. The PLBs showed much lower cellular adhesion than bare silicon controls. Of the cells that attached to the PLBs, a very low percentage showed the same morphological expressions seen on the controls. It is hypothesized that proteins adsorb to the defects in the PLBs, caused by incomplete polymerization, and this mediates the observed minimal cellular attachment and morphological changes.

Original languageEnglish (US)
Pages (from-to)212-217
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume97 A
Issue number2
DOIs
StatePublished - May 2011

Keywords

  • RAW 264.7
  • bis-SorbPC
  • host response
  • macrophage
  • polymerized lipid bilayers

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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