In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies

Renu Bansal, Nafees Ahmad, Jalil R. Kidwai

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of14C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.

Original languageEnglish (US)
Pages (from-to)255-266
Number of pages12
JournalActa Diabetologica Latina
Volume17
Issue number3-4
DOIs
StatePublished - Jul 1 1980
Externally publishedYes

    Fingerprint

Keywords

  • Cathepsin B
  • Cathepsin B antibodies
  • Islets of Langerhans
  • Proinsulin-insulin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this