In vitro intestinal processing of prostaglandin F(2α) in rats

Developmental aspects

Alan D Bedrick, S. M. Johnson, O. Koldovsky

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cytoprotective prostaglandins (PGs) such as PGF(2α) are present in milk and can be absorbed intact from the gastrointestinal tract. Developmental differences in gastrointestinal PG metabolism in vivo have been previously reported. To evaluate the role of small intestinal tissue, detailed analysis of PG processing in vitro of small intestinal segments of suckling and weanling rats using everted sacs was performed. Sacs were incubated in Krebs-Ringer bicarbonate buffer, pH 7.4, at 37°C. Bathing mucosal fluid contained [3H]PGF(2α). Proximal and middle intestinal segment everted sacs of suckling rats exhibited greater transfer of radioactivity in comparison with those of weanling rats. Whereas in suckling rats transfer of radioactivity decreased from proximal to distal regions of the intestine, no segmental differences were seen in weanlings. Maturational differences were also present in the capacity for PG catabolism. Suckling rat everted sacs had a greater proportion of radioactivity present as intact, unmetabolized PGF(2α) present in sac tissue and serosal fluid in all intestinal segments in comparison with those of weanling rats. Weanling everted sacs contained greater amounts of less polar metabolites. These results indicate that there are quantitative and qualitative age-related differences in transfer and processing of exogenous PGF(2α) in small intestinal tissue of developing rats.

Original languageEnglish (US)
Pages (from-to)755-761
Number of pages7
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume7
Issue number5
StatePublished - 1988

Fingerprint

Prostaglandins F
prostaglandins
weanlings
suckling
rats
Prostaglandins
Radioactivity
tissue analysis
metabolism
Peritoneal Cavity
Bicarbonates
In Vitro Techniques
bicarbonates
gastrointestinal system
Intestines
Gastrointestinal Tract
Buffers
Milk
intestines
buffers

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Gastroenterology
  • Histology
  • Pediatrics, Perinatology, and Child Health

Cite this

In vitro intestinal processing of prostaglandin F(2α) in rats : Developmental aspects. / Bedrick, Alan D; Johnson, S. M.; Koldovsky, O.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 7, No. 5, 1988, p. 755-761.

Research output: Contribution to journalArticle

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