In vitro osmoregulation of taurine in fetal mouse hearts

Matthew Atlas, Joseph John Bahl, William R Roeske, Rubin Bressler

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Regulation of taurine transport and accumulation in explanted fetal mouse hearts is shown to be under osmotic control. All osmotic agents studied, both ionic (NaCl, LiCl, choline Cl) and nonionic (sucrose, glucose) stimulated [3H]-taurine transport during an incubation of 19 h. Hyperosmotic stimulation of transport achieved statistical significance by 3 h in the presence of sucrose (P<0.05). After 1 h, 40 mm naCl engendered a 56% increase in [3H]-taurine transport (P<0.01). The NaCl stimulation at 1 h may relate more to the transport system's absolute sodium ion requirement than hyperosmotic stimulation. Incremental addition of NaCl or sucrose linearly stimulates [3H]-taurine transport in an incubation of 19 h. Total taurine, measured by HPLC, increased 25% with addition of either 40 mm NaCl or 80 mm sucrose. Hyperosmotic stimulation of transport was not blocked with propranolol but was additive to β-adrenergic stimulation of transport. Osmotic change in Km (0.51→0.43 mm). After 1 h preincubation with a hypersomotic addition phenylalanine transport was measured, but was not different from control. Phenylalanine accumulation measured during 19 h incubation similarly was not altered. Streptozotocin induced diabetic rats had elevated plasma osmolarities (295±2.1→322±1.3 mosmol) and cardiac taurine (24.3±1.2→36±1.0μmol/g wet wt.). The data presented demonstrates that mammalian cardiac taurine is regulated by the osmotic environment of the heart, suggesting an osmoregulatory function for intracellular taurine and physiological relevance in disease states such as diabetes.

Original languageEnglish (US)
Pages (from-to)311-320
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume16
Issue number4
DOIs
StatePublished - 1984

Fingerprint

Osmoregulation
Fetal Heart
Taurine
Sucrose
Phenylalanine
In Vitro Techniques
Streptozocin
Choline
Propranolol
Adrenergic Agents
Osmolar Concentration
Sodium
High Pressure Liquid Chromatography
Ions
Glucose

Keywords

  • Diabetes
  • Osmoregulation
  • Taurine
  • Transport

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

In vitro osmoregulation of taurine in fetal mouse hearts. / Atlas, Matthew; Bahl, Joseph John; Roeske, William R; Bressler, Rubin.

In: Journal of Molecular and Cellular Cardiology, Vol. 16, No. 4, 1984, p. 311-320.

Research output: Contribution to journalArticle

Atlas, Matthew ; Bahl, Joseph John ; Roeske, William R ; Bressler, Rubin. / In vitro osmoregulation of taurine in fetal mouse hearts. In: Journal of Molecular and Cellular Cardiology. 1984 ; Vol. 16, No. 4. pp. 311-320.
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AB - Regulation of taurine transport and accumulation in explanted fetal mouse hearts is shown to be under osmotic control. All osmotic agents studied, both ionic (NaCl, LiCl, choline Cl) and nonionic (sucrose, glucose) stimulated [3H]-taurine transport during an incubation of 19 h. Hyperosmotic stimulation of transport achieved statistical significance by 3 h in the presence of sucrose (P<0.05). After 1 h, 40 mm naCl engendered a 56% increase in [3H]-taurine transport (P<0.01). The NaCl stimulation at 1 h may relate more to the transport system's absolute sodium ion requirement than hyperosmotic stimulation. Incremental addition of NaCl or sucrose linearly stimulates [3H]-taurine transport in an incubation of 19 h. Total taurine, measured by HPLC, increased 25% with addition of either 40 mm NaCl or 80 mm sucrose. Hyperosmotic stimulation of transport was not blocked with propranolol but was additive to β-adrenergic stimulation of transport. Osmotic change in Km (0.51→0.43 mm). After 1 h preincubation with a hypersomotic addition phenylalanine transport was measured, but was not different from control. Phenylalanine accumulation measured during 19 h incubation similarly was not altered. Streptozotocin induced diabetic rats had elevated plasma osmolarities (295±2.1→322±1.3 mosmol) and cardiac taurine (24.3±1.2→36±1.0μmol/g wet wt.). The data presented demonstrates that mammalian cardiac taurine is regulated by the osmotic environment of the heart, suggesting an osmoregulatory function for intracellular taurine and physiological relevance in disease states such as diabetes.

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