In vitro ovarian tissue and organ culture: a review.

P. J. Devine, K. S. Rajapaksa, Patricia B Hoyer

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Many investigations have utilized techniques for culturing ovarian tissue or isolated follicles in vitro. Whole ovaries from fetal or neonatal rodents have been incubated in organ culture systems. This has been utilized to understand the sequence of follicle formation and its hormonal requirements, activation of quiescent follicles, follicular growth and development, and acquisition of steroidogenic capabilities. Adaptations of this technique include incubation of ovaries in a chamber continuously perfused with medium or perfusion of medium through the intact vasculature. Late follicular development, ovulation, and steroidogenesis can also be examined in these systems. Another approach has been to culture individual follicles isolated by enzymatic or mechanical dissociation. The majority of this research has focused on improving follicular development in vitro. This review will discuss these various culture techniques and some of the results that have been acquired. Recent results from toxicological studies utilizing whole ovarian cultures performed will also be described. Future applications for ovarian and follicular cultures may include in vitro follicular development for eventual production of offspring from frozen ovarian tissue, mechanistic studies related to the impact of endocrine disruptors and ovotoxicants on ovarian function, and further investigations into follicle activation and development.

Original languageEnglish (US)
JournalFrontiers in Bioscience
Volume7
StatePublished - 2002

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Organ Culture Techniques
Chemical activation
Tissue
Endocrine Disruptors
Ovary
Culture Techniques
Ovulation
Growth and Development
Toxicology
Rodentia
Perfusion
Research
In Vitro Techniques

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In vitro ovarian tissue and organ culture : a review. / Devine, P. J.; Rajapaksa, K. S.; Hoyer, Patricia B.

In: Frontiers in Bioscience, Vol. 7, 2002.

Research output: Contribution to journalArticle

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