In vitro pro-inflammatory regulatory role of substance P in alveolar macrophages and type II pneumocytes after JP-8 exposure

Nina N. Sun, Simon S. Wong, Celine Nardi, Daniel Ostroff, Mark L. Witten, Robert Clark Lantz

Research output: Contribution to journalArticle

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Abstract

The effects of JP-8 on pro-inflammatory cytokine interleukin (IL)-1α,β and nitric oxide (NO) secretion as well as the role of substance P (SP) in these processes were examined in cultured alveolar macrophages (AM), type II epithelial cells (AIIE), and AM/AIIE co-cultures. Exposure of AM to JP-8 for 24 hr exhibited release of IL-1α,β, whereas exposure to AIIE showed a concentration-dependent NO overproduction. Data indicate that there are cell-dependent inflammatory mechanisms responsible for the actual level of JP-8 exposure in alveoli. However, treatment with substance P significantly attenuated JP-8 induced the IL-1α,β secretion. This finding was confirmed by using [Sar9 Met (O2)11] SP (10- 10 M), an agonist of substance P, suggesting that substance P may have signal pathway(s) to AM in the inflammatory response mediated by IL-1. Moreover, AM/AIIE co-culture obviously reduced NO overproduction observed in AIIE alone, suggesting that there may be cell interactions or communications between AM and AIIE in response to the JP-8 exposure.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalJournal of Immunotoxicology
Volume4
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Alveolar Epithelial Cells
Alveolar Macrophages
Substance P
Epithelial Cells
Interleukin-1
Nitric Oxide
Coculture Techniques
Cell Communication
Cell Culture Techniques
In Vitro Techniques
Signal Transduction
Cytokines
Communication

Keywords

  • Co-cultures
  • Cytokines
  • JP-8
  • Macrophages
  • Nitric oxide
  • Type II pneumocytes

ASJC Scopus subject areas

  • Toxicology
  • Immunology
  • Immunology and Allergy

Cite this

In vitro pro-inflammatory regulatory role of substance P in alveolar macrophages and type II pneumocytes after JP-8 exposure. / Sun, Nina N.; Wong, Simon S.; Nardi, Celine; Ostroff, Daniel; Witten, Mark L.; Lantz, Robert Clark.

In: Journal of Immunotoxicology, Vol. 4, No. 1, 01.2007, p. 61-67.

Research output: Contribution to journalArticle

Sun, Nina N. ; Wong, Simon S. ; Nardi, Celine ; Ostroff, Daniel ; Witten, Mark L. ; Lantz, Robert Clark. / In vitro pro-inflammatory regulatory role of substance P in alveolar macrophages and type II pneumocytes after JP-8 exposure. In: Journal of Immunotoxicology. 2007 ; Vol. 4, No. 1. pp. 61-67.
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