In vitro pro-inflammatory regulatory role of substance P in alveolar macrophages and type II pneumocytes after JP-8 exposure

Nina N. Sun, Simon S. Wong, Celine Nardi, Daniel Ostroff, Mark L. Witten, R. Clark Lantz

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effects of JP-8 on pro-inflammatory cytokine interleukin (IL)-1α,β and nitric oxide (NO) secretion as well as the role of substance P (SP) in these processes were examined in cultured alveolar macrophages (AM), type II epithelial cells (AIIE), and AM/AIIE co-cultures. Exposure of AM to JP-8 for 24 hr exhibited release of IL-1α,β, whereas exposure to AIIE showed a concentration-dependent NO overproduction. Data indicate that there are cell-dependent inflammatory mechanisms responsible for the actual level of JP-8 exposure in alveoli. However, treatment with substance P significantly attenuated JP-8 induced the IL-1α,β secretion. This finding was confirmed by using [Sar9 Met (O2)11] SP (10- 10 M), an agonist of substance P, suggesting that substance P may have signal pathway(s) to AM in the inflammatory response mediated by IL-1. Moreover, AM/AIIE co-culture obviously reduced NO overproduction observed in AIIE alone, suggesting that there may be cell interactions or communications between AM and AIIE in response to the JP-8 exposure.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalJournal of Immunotoxicology
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Co-cultures
  • Cytokines
  • JP-8
  • Macrophages
  • Nitric oxide
  • Type II pneumocytes

ASJC Scopus subject areas

  • Immunology
  • Toxicology

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