In vitro response of v-Ha-ras transgenic mouse lymphocytes after in vivo treatment with cocaine

Lucas L. Colombo, María C. López, GuanJie Chen, Ronald R Watson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Oncomouse is a transgenic mouse carrying an activated v-Ha-ras oncogene under the control of the mouse mammary tumor virus promoter. The objective of this paper was to learn if the in vitro secretion of IL-2 and IFN-γ and the release of sIL-2R by Oncomice spleen and thymus cells depended on the presence of the oncogene product, on the in vivo pretreatment with cocaine, or on the in vitro treatment with cocaine or morphine. Oncomice thymocytes from different experimental groups released less sIL-2R than FVB thymocytes. Oncornice thymocytes secreted more IFN-γ than FVB thymocytes. Oncomice thymocytes cultured in the presence of Con A and cocaine showed a diminished release of sIL-2R and a lower secretion of IFN-γ, a phenomenon not observed in FVB thymocytes. IFN-γ secretion was lower in Oncomice splenocytes. In general, Oncomice thymocytes and splenocytes responded in a nearly opposite fashion to their FVB counterparts. In this study, the in vitro response to mitogens, cocaine or morphine depended on genetic background and not on the in vivo pretreatment with cocaine. Our results emphasize the role of the v-Ha-ras oncogene in defining the host immune response.

Original languageEnglish (US)
Pages (from-to)607-616
Number of pages10
JournalImmunopharmacology and Immunotoxicology
Volume23
Issue number4
DOIs
StatePublished - 2001

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Lymphocytes
Thymocytes
Cocaine
Transgenic Mice
Morphine
ras Genes
Thymus
Oncogene Proteins
Viruses
Mitogens
Mouse mammary tumor virus
Interleukin-2
Tumors
In Vitro Techniques
Carcinogens
Thymus Gland
Spleen

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

In vitro response of v-Ha-ras transgenic mouse lymphocytes after in vivo treatment with cocaine. / Colombo, Lucas L.; López, María C.; Chen, GuanJie; Watson, Ronald R.

In: Immunopharmacology and Immunotoxicology, Vol. 23, No. 4, 2001, p. 607-616.

Research output: Contribution to journalArticle

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