In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants

Nadia Khelef, David DeShazer, Richard L Friedman, Nicole Guiso

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed 5774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalFEMS Microbiology Letters
Volume142
Issue number2-3
DOIs
StatePublished - Sep 1 1996

Fingerprint

Bordetella pertussis
Catalase
Superoxide Dismutase
Respiratory Tract Infections
Virulence
Adenylate Cyclase Toxin
Neutrophils
Macrophages
Apoptosis
Bacteria
Enzymes
In Vitro Techniques

Keywords

  • Bordetella pertussis
  • Catalase
  • Superoxide dismutase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants. / Khelef, Nadia; DeShazer, David; Friedman, Richard L; Guiso, Nicole.

In: FEMS Microbiology Letters, Vol. 142, No. 2-3, 01.09.1996, p. 231-235.

Research output: Contribution to journalArticle

Khelef, Nadia ; DeShazer, David ; Friedman, Richard L ; Guiso, Nicole. / In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants. In: FEMS Microbiology Letters. 1996 ; Vol. 142, No. 2-3. pp. 231-235.
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