In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants

Nadia Khelef, David DeShazer, Richard L. Friedman, Nicole Guiso

Research output: Contribution to journalArticle

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Abstract

Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed 5774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalFEMS Microbiology Letters
Volume142
Issue number2-3
DOIs
StatePublished - Sep 1 1996

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Keywords

  • Bordetella pertussis
  • Catalase
  • Superoxide dismutase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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