In vivo electrochemical measurements of exogenously applied dopamine in Drosophila melanogaster

Monique A. Makos, Young Cho Kim, Kyung An Han, Michael L Heien, Andrew G. Ewing

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Carbon-fiber microelectrodes coupled with electrochemical detection have been used extensively for the analysis of biogenic amines. In order to determine the functional role of these amines, in vivo studies have primarily used rats and mice as model organisms. Here, we report on the development of these microanalytical techniques for in vivo electrochemical detection of dopamine in the adult Drosophila melanogastercentral nervous system (CNS). A triple-barrel micropipet injector was used to exogenously apply three different concentrations of dopamine, and a cylindrical carbon-fiber microelectrode was placed in the protocerebral anterior medial brain area where dopamine neurons are densely populated. Background- subtracted fast-scan cyclic voltammetry was used to measure dopamine concentration in the fly CNS. Distinct differences are shown for the clearance of exogenously applied dopamine in the brains of wild type flies versus fumin (fmn) mutants lacking a functional dopamine transporter. The current response due to oxidation of dopamine increased significantly from baseline for wild type flies following cocaine incubation. Interestingly, the current remained unchanged for mutant flies under the same conditions. These data confirm the accepted theory that cocaine blocks dopamine transporter function and validates the use of in vivo electrochemical methods to monitor dopamine uptake in Drosophila. Furthermore, after incubation with tetrodotoxin (TTX), a sodium channel blocker, there was a significant increase in peak oxidation current in the wild type flies; however, the current did not significantly change in the fmn mutant. These data suggest that factors that affect neuronal activity via ion channels such as TTX also influence the function of the dopamine transporter in Drosophila.

Original languageEnglish (US)
Pages (from-to)1848-1854
Number of pages7
JournalAnalytical Chemistry
Volume81
Issue number5
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

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Dopamine
Dopamine Plasma Membrane Transport Proteins
Microelectrodes
Tetrodotoxin
Cocaine
Brain
Sodium Channel Blockers
Oxidation
Biogenic Amines
Neurology
Ion Channels
Cyclic voltammetry
Neurons
Amines
Rats

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

In vivo electrochemical measurements of exogenously applied dopamine in Drosophila melanogaster. / Makos, Monique A.; Kim, Young Cho; Han, Kyung An; Heien, Michael L; Ewing, Andrew G.

In: Analytical Chemistry, Vol. 81, No. 5, 01.03.2009, p. 1848-1854.

Research output: Contribution to journalArticle

Makos, Monique A. ; Kim, Young Cho ; Han, Kyung An ; Heien, Michael L ; Ewing, Andrew G. / In vivo electrochemical measurements of exogenously applied dopamine in Drosophila melanogaster. In: Analytical Chemistry. 2009 ; Vol. 81, No. 5. pp. 1848-1854.
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