In vivo endoscopic Doppler optical coherence tomography imaging of mouse colon

Weston A. Welge, Jennifer K Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Colorectal cancer remains the second deadliest cancer in the United States, despite the high sensitivity and specificity of colonoscopy and sigmoidoscopy. While these standard imaging procedures can accurately detect medium and large polyps, some studies have shown miss rates up to 25% for polyps less than 5 mm in diameter. An imaging modality capable of detecting small lesions could potentially improve patient outcomes. Optical coherence tomography (OCT) has been shown to be a powerful imaging modality for adenoma detection in a mouse model of colorectal cancer. While previous work has focused on analyzing the structural OCT images based on thickening of the mucosa and changes in light attenuation in depth, imaging the microvasculature of the colon may enable earlier detection of polyps. The structure and function of vessels grown to support tumor growth are markedly different from healthy vessels. Doppler OCT is capable of imaging microvessels in vivo. We developed a method of processing raw fringe data from a commercial swept-source OCT system using a lab-built miniature endoscope to extract microvessels. This method can be used to measure vessel count and density and to measure flow velocities. This may improve early detection and aid in the development of new chemopreventive and chemotherapeutic drugs. We present, to the best of our knowledge, the first endoscopic Doppler OCT images of in vivo mouse colon.

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
PublisherSPIE
Volume9691
ISBN (Electronic)9781628419252
DOIs
StatePublished - 2016
EventEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III - San Francisco, United States
Duration: Feb 14 2016Feb 15 2016

Other

OtherEndoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III
CountryUnited States
CitySan Francisco
Period2/14/162/15/16

Keywords

  • adenoma
  • colorectal cancer
  • Doppler optical coherence tomography
  • endoscopy
  • image processing

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Welge, W. A., & Barton, J. K. (2016). In vivo endoscopic Doppler optical coherence tomography imaging of mouse colon. In Endoscopic Microscopy XI; and Optical Techniques in Pulmonary Medicine III (Vol. 9691). [96910T] SPIE. https://doi.org/10.1117/12.2211954