In-vivo fluorescence spectroscopy of normal and atherosclerotic arteries

Lawrence I. Deckelbaum, Ian J. Sarembock, Mark L. Stetz, Kenneth M. O'Brien, Francis W. Cutruzzola, Arthur F Gmitro, Michael D. Ezekowitz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

Laser-induced fluorescence spectroscopy can discriminate atherosclerotic from normal arteries in-vitro and may thus potentially guide laser angioplasty. To evaluate the feasibility of laser-induced fluorescence spectroscopy in a living blood-filled arterial system we performed fiberoptic laser-induced fluorescence spectroscopy in a rabbit model of focal femoral atherosclerosis. A laser-induced fluorescence spectroscopy score was derived from stepwise linear regression analysis of in-vitro spectra to distinguish normal aorta (score>0) from atherosclerotic femoral artery (score<0). A 400 u silica fiber, coupled to a helium cadmium laser and optical multichannel analyzer, was inserted through a 5F catheter to induce and record in-vivo fluorescence from femoral and aortoiliac arteries. Arterial spectra could be recorded in all animals (n=10: 5 occlusions, 5 stenoses). Blood spectra were of low intensity and were easily distinguished from arterial spectra. The scores (mean ± SEM) for the in-vivo spectra were -0.69 ± 0.29 for artherosclerotic femoral, and +0.54 ±. 0.15 for normal aorta (p<.01; p=NS compared to in-vitro spectra). In-vitro, a fiber tip to tissue distance <50 u was necessary for adequate arterial LIFS in blood. At larger distances low intensity blood spectra were recorded (1/20 the intensity of tissue spectra). Thus, fiberoptic laser-induced fluorescence spectroscopy can be sucessfully performed in a blood filled artery provided the fiber tip is approximated to the tissue.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages314-319
Number of pages6
Volume906
DOIs
Publication statusPublished - 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Deckelbaum, L. I., Sarembock, I. J., Stetz, M. L., O'Brien, K. M., Cutruzzola, F. W., Gmitro, A. F., & Ezekowitz, M. D. (1988). In-vivo fluorescence spectroscopy of normal and atherosclerotic arteries. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 906, pp. 314-319) https://doi.org/10.1117/12.945298