In-vivo fluorescence spectroscopy of normal and atherosclerotic arteries

Lawrence I. Deckelbaum, Ian J. Sarembock, Mark L. Stetz, Kenneth M. O’brien, Francis W. Cutruzzola, Arthur F. Gmitro, Michael D. Ezekowitz

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Laser-induced fluorescence spectroscopy can discriminate atherosclerotic from normal arteries in-vitro and may thus potentially guide laser angioplasty. To evaluate the feasibility of laser-induced fluorescence spectroscopy in a living blood-filled arterial system we performed fiberoptic laser-induced fluorescence spectroscopy in a rabbit model of focal femoral atherosclerosis. A laser-induced fluorescence spectroscopy score was derived from stepwise linear regression analysis of in-vitro spectra to distinguish normal aorta (score>0) from atherosclerotic femoral artery (score<0). A 400 u silica fiber, coupled to a helium cadmium laser and optical multichannel analyzer, was inserted through a 5F catheter to induce and record in-vivo fluorescence from femoral and aortoiliac arteries. Arterial spectra could be recorded in all animals (n=10: 5 occlusions, 5 stenoses). Blood spectra were of low intensity and were easily distinguished from arterial spectra. The scores (mean ± SEM) for the in-vivo spectra were −0.69 ± 0.29 for artherosclerotic femoral, and +0.54 ±. 0.15 for normal aorta (p<.01; p=NS compared to in-vitro spectra). In-vitro, a fiber tip to tissue distance <50 u was necessary for adequate arterial LIFS in blood. At larger distances low intensity blood spectra were recorded (1/20 the intensity of tissue spectra). Thus, fiberoptic laser-induced fluorescence spectroscopy can be sucessfully performed in a blood filled artery provided the fiber tip is approximated to the tissue.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume906
DOIs
StatePublished - Jun 21 1988
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Deckelbaum, L. I., Sarembock, I. J., Stetz, M. L., O’brien, K. M., Cutruzzola, F. W., Gmitro, A. F., & Ezekowitz, M. D. (1988). In-vivo fluorescence spectroscopy of normal and atherosclerotic arteries. Proceedings of SPIE - The International Society for Optical Engineering, 906, 314-319. https://doi.org/10.1117/12.945298