In vivo imaging of inflammatory responses by photoacoustics using cell-targeted gold nanorods (GNR) as contrast agent

K. Kim, A. Agarwal, A. M. Mcdonald, R. M. Moore, D. D. Myers, R. S. Witte, S. W. Huang, S. Ashkenazi, M. J. Kaplan, T. W. Wakefield, M. O'Donnell, N. A. Kotov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

Cardiovascular inflammatory activity was imaged in vivo. Inflammation is known to be a major cause of cardiovascular disease. Photoacoustic (PA) imaging was employed using bio-conjugated gold nanorods (GNR) as a contrast agent. A mouse model based on vascular endothelium injury by a photochemical reaction of Rose Bengal (RB) dye to green light laser was used. Following a mid-line laparotomy under an approved animal protocol, anti-ICAM-1 conjugated GNR was injected through the dorsal penile vein followed by RB injection through the same vein. The inferior vena cava immediately distal to the renal veins of a C57BL/6 mouse was exposed to the green light laser for 10 minutes. The peak absorption of GNR was tuned to be 700 nm to minimize possible background absorption by blood and RB. The stability of GNR in the blood plasma was tested in vitro. Photoacoustic images were obtained through an ultrasound gel pouch in the mouse abdomen using a commercial ultrasound probe to evaluate inflammatory changes to the vascular endothelium, confirmed by histology. Preliminary results demonstrate the feasibility of in vivo photoacoustic imaging by a commercial ultrasound scanner of inflammation using GNR as a contrast agent.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
DOIs
StatePublished - Apr 21 2008
Event9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008 - San Jose, CA, United States
Duration: Jan 20 2008Jan 23 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6856
ISSN (Print)1605-7422

Other

Other9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
CountryUnited States
CitySan Jose, CA
Period1/20/081/23/08

Keywords

  • Gold nanorods
  • Inflammation
  • Molecular imaging
  • Mouse imaging
  • Photoacoustics
  • Vascular injury

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Kim, K., Agarwal, A., Mcdonald, A. M., Moore, R. M., Myers, D. D., Witte, R. S., Huang, S. W., Ashkenazi, S., Kaplan, M. J., Wakefield, T. W., O'Donnell, M., & Kotov, N. A. (2008). In vivo imaging of inflammatory responses by photoacoustics using cell-targeted gold nanorods (GNR) as contrast agent. In Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics [68560H] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6856). https://doi.org/10.1117/12.763498