In-vivo multi-spectral confocal microscopy

Andrew R Rouse, Joshua A. Udovich, Arthur F Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

A multi-spectral confocal microendoscope (MCME) for in-vivo imaging has been developed. The MCME employs a flexible fiber-optic catheter coupled to a slit-scan confocal microscope with an imaging spectrometer. The catheter consists of a fiber-optic imaging bundle linked to a miniature objective and focus assembly. The focus mechanism allows for imaging to a maximum tissue depth of 200 microns. The 3mm diameter catheter may be used on its own or routed though the instrument channel of a commercial endoscope. The confocal nature of the system provides optical sectioning with 3 micron lateral resolution and 30 micron axial resolution. The system incorporates two laser sources and is therefore capable of simultaneous acquisition of spectra from multiple dyes using dual excitation. The prism based multi-spectral detection assembly is typically configured to collect 30 spectral samples over the visible range. The spectral sampling rate varies from 4nm/pixel at 490nm to 8nm/pixel at 660nm and the minimum resolvable wavelength difference varies from 8nm to 16nm over the same spectral range. Each of these characteristics are primarily dictated by the dispersion characteristics of the prism. The MCME is designed to examine cellular structures during optical biopsy and to exploit the diagnostic information contained within the spectral domain. The primary applications for the system include diagnosis of disease in the gastro-intestinal tract and female reproductive system. In-vitro, and ex-vivo multi-spectral results are presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsJ.-A. Conchello, C.J. Cogswell, T. Wilson
Pages73-84
Number of pages12
Volume5701
DOIs
StatePublished - 2005
EventThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII - San Jose, CA, United States
Duration: Jan 25 2005Jan 27 2005

Other

OtherThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII
CountryUnited States
CitySan Jose, CA
Period1/25/051/27/05

Fingerprint

Confocal microscopy
Catheters
Imaging techniques
Prisms
Fiber optics
Pixels
Endoscopy
Biopsy
Optical systems
Spectrometers
Microscopes
Dyes
Tissue
Sampling
Wavelength
Lasers

Keywords

  • Confocal
  • Endoscope
  • Fiber
  • Fluorescence
  • Microscopy
  • Optics
  • Scan
  • Spectroscopy
  • Tissue

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Rouse, A. R., Udovich, J. A., & Gmitro, A. F. (2005). In-vivo multi-spectral confocal microscopy. In J-A. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5701, pp. 73-84). [13] https://doi.org/10.1117/12.591097

In-vivo multi-spectral confocal microscopy. / Rouse, Andrew R; Udovich, Joshua A.; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / J.-A. Conchello; C.J. Cogswell; T. Wilson. Vol. 5701 2005. p. 73-84 13.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rouse, AR, Udovich, JA & Gmitro, AF 2005, In-vivo multi-spectral confocal microscopy. in J-A Conchello, CJ Cogswell & T Wilson (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5701, 13, pp. 73-84, Three- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII, San Jose, CA, United States, 1/25/05. https://doi.org/10.1117/12.591097
Rouse AR, Udovich JA, Gmitro AF. In-vivo multi-spectral confocal microscopy. In Conchello J-A, Cogswell CJ, Wilson T, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5701. 2005. p. 73-84. 13 https://doi.org/10.1117/12.591097
Rouse, Andrew R ; Udovich, Joshua A. ; Gmitro, Arthur F. / In-vivo multi-spectral confocal microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / J.-A. Conchello ; C.J. Cogswell ; T. Wilson. Vol. 5701 2005. pp. 73-84
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