In-vivo multi-spectral confocal microscopy

Andrew R. Rouse, Joshua A. Udovich, Arthur F. Gmitro

Research output: Contribution to journalConference article

5 Scopus citations

Abstract

A multi-spectral confocal microendoscope (MCME) for in-vivo imaging has been developed. The MCME employs a flexible fiber-optic catheter coupled to a slit-scan confocal microscope with an imaging spectrometer. The catheter consists of a fiber-optic imaging bundle linked to a miniature objective and focus assembly. The focus mechanism allows for imaging to a maximum tissue depth of 200 microns. The 3mm diameter catheter may be used on its own or routed though the instrument channel of a commercial endoscope. The confocal nature of the system provides optical sectioning with 3 micron lateral resolution and 30 micron axial resolution. The system incorporates two laser sources and is therefore capable of simultaneous acquisition of spectra from multiple dyes using dual excitation. The prism based multi-spectral detection assembly is typically configured to collect 30 spectral samples over the visible range. The spectral sampling rate varies from 4nm/pixel at 490nm to 8nm/pixel at 660nm and the minimum resolvable wavelength difference varies from 8nm to 16nm over the same spectral range. Each of these characteristics are primarily dictated by the dispersion characteristics of the prism. The MCME is designed to examine cellular structures during optical biopsy and to exploit the diagnostic information contained within the spectral domain. The primary applications for the system include diagnosis of disease in the gastro-intestinal tract and female reproductive system. In-vitro, and ex-vivo multi-spectral results are presented.

Original languageEnglish (US)
Article number13
Pages (from-to)73-84
Number of pages12
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5701
DOIs
StatePublished - Jul 21 2005
EventThree- Dimensional and Multidomensional Microscopy: Image Acquisition and Processing XII - San Jose, CA, United States
Duration: Jan 25 2005Jan 27 2005

Keywords

  • Confocal
  • Endoscope
  • Fiber
  • Fluorescence
  • Microscopy
  • Optics
  • Scan
  • Spectroscopy
  • Tissue

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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