In vivo rabbit eyecup preparation for use in retinal research

Miltiadis Tsilimbaris, Eugene S. Lit, John B Christoforidis, Donald J. D'Amico

Research output: Contribution to journalArticle

Abstract

PURPOSE. To develop an in vivo rabbit eyecup preparation that preserves neuronal and vascular connections with normal posterior segment contour, permitting direct access to the retina and facilitating retinal microsurgical and neuroscience research. METHODS. Cyanoacrylate glue was applied to the anterior sclera of six Dutch-belted rabbits before open-sky vitrectomy. The glue was used to harden the compliant scleral wall and to fix it to the surrounding periorbital tissues. RESULTS. A normal contour of the posterior segment was retained in all cases. Vitrectomy under air was successfully accomplished and an extensive removal of the vitreous gel was achieved. Fluorescein angiography revealed normal vascular patency of the retinal vessels after vitrectomy. CONCLUSIONS. The proposed modification of the rabbit eyecup retains the normal neurovascular connections and configuration of the retina, making it suitable for retinal microsurgical maneuvers or any procedures in which easy access to anatomically undisturbed retina is required.

Original languageEnglish (US)
Pages (from-to)906-909
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Vitrectomy
Retina
Rabbits
Adhesives
Vascular Patency
Research
Cyanoacrylates
Retinal Vessels
Sclera
Fluorescein Angiography
Neurosciences
Blood Vessels
Gels
Air

ASJC Scopus subject areas

  • Ophthalmology

Cite this

In vivo rabbit eyecup preparation for use in retinal research. / Tsilimbaris, Miltiadis; Lit, Eugene S.; Christoforidis, John B; D'Amico, Donald J.

In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 3, 03.2004, p. 906-909.

Research output: Contribution to journalArticle

Tsilimbaris, Miltiadis ; Lit, Eugene S. ; Christoforidis, John B ; D'Amico, Donald J. / In vivo rabbit eyecup preparation for use in retinal research. In: Investigative Ophthalmology and Visual Science. 2004 ; Vol. 45, No. 3. pp. 906-909.
@article{682e0585b975435fa2c31a7ed381053c,
title = "In vivo rabbit eyecup preparation for use in retinal research",
abstract = "PURPOSE. To develop an in vivo rabbit eyecup preparation that preserves neuronal and vascular connections with normal posterior segment contour, permitting direct access to the retina and facilitating retinal microsurgical and neuroscience research. METHODS. Cyanoacrylate glue was applied to the anterior sclera of six Dutch-belted rabbits before open-sky vitrectomy. The glue was used to harden the compliant scleral wall and to fix it to the surrounding periorbital tissues. RESULTS. A normal contour of the posterior segment was retained in all cases. Vitrectomy under air was successfully accomplished and an extensive removal of the vitreous gel was achieved. Fluorescein angiography revealed normal vascular patency of the retinal vessels after vitrectomy. CONCLUSIONS. The proposed modification of the rabbit eyecup retains the normal neurovascular connections and configuration of the retina, making it suitable for retinal microsurgical maneuvers or any procedures in which easy access to anatomically undisturbed retina is required.",
author = "Miltiadis Tsilimbaris and Lit, {Eugene S.} and Christoforidis, {John B} and D'Amico, {Donald J.}",
year = "2004",
month = "3",
doi = "10.1167/iovs.03-0414",
language = "English (US)",
volume = "45",
pages = "906--909",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - In vivo rabbit eyecup preparation for use in retinal research

AU - Tsilimbaris, Miltiadis

AU - Lit, Eugene S.

AU - Christoforidis, John B

AU - D'Amico, Donald J.

PY - 2004/3

Y1 - 2004/3

N2 - PURPOSE. To develop an in vivo rabbit eyecup preparation that preserves neuronal and vascular connections with normal posterior segment contour, permitting direct access to the retina and facilitating retinal microsurgical and neuroscience research. METHODS. Cyanoacrylate glue was applied to the anterior sclera of six Dutch-belted rabbits before open-sky vitrectomy. The glue was used to harden the compliant scleral wall and to fix it to the surrounding periorbital tissues. RESULTS. A normal contour of the posterior segment was retained in all cases. Vitrectomy under air was successfully accomplished and an extensive removal of the vitreous gel was achieved. Fluorescein angiography revealed normal vascular patency of the retinal vessels after vitrectomy. CONCLUSIONS. The proposed modification of the rabbit eyecup retains the normal neurovascular connections and configuration of the retina, making it suitable for retinal microsurgical maneuvers or any procedures in which easy access to anatomically undisturbed retina is required.

AB - PURPOSE. To develop an in vivo rabbit eyecup preparation that preserves neuronal and vascular connections with normal posterior segment contour, permitting direct access to the retina and facilitating retinal microsurgical and neuroscience research. METHODS. Cyanoacrylate glue was applied to the anterior sclera of six Dutch-belted rabbits before open-sky vitrectomy. The glue was used to harden the compliant scleral wall and to fix it to the surrounding periorbital tissues. RESULTS. A normal contour of the posterior segment was retained in all cases. Vitrectomy under air was successfully accomplished and an extensive removal of the vitreous gel was achieved. Fluorescein angiography revealed normal vascular patency of the retinal vessels after vitrectomy. CONCLUSIONS. The proposed modification of the rabbit eyecup retains the normal neurovascular connections and configuration of the retina, making it suitable for retinal microsurgical maneuvers or any procedures in which easy access to anatomically undisturbed retina is required.

UR - http://www.scopus.com/inward/record.url?scp=1542742176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542742176&partnerID=8YFLogxK

U2 - 10.1167/iovs.03-0414

DO - 10.1167/iovs.03-0414

M3 - Article

VL - 45

SP - 906

EP - 909

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -