Incorporating Fast Protein Dynamics into Enzyme Design: A Proposed Mutant Aromatic Amine Dehydrogenase

Ioanna Zoi, Dimitri Antoniou, Steven D Schwartz

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

In recent years, there has been encouraging progress in the engineering of enzymes that are designed to catalyze reactions not accelerated by natural enzymes. We tested the possibility of reengineering an existing enzyme by introducing a fast protein motion that couples to the reaction. Aromatic amine dehydrogenase is a system that has been shown to use a fast substrate motion as part of the reaction mechanism. We identified a mutation that preserves this fast motion but also introduces a favorable fast motion near the active site that did not exist in the native enzyme. Transition path sampling was used for the analysis of the atomic details of the mechanism.

Original languageEnglish (US)
Pages (from-to)7290-7298
Number of pages9
JournalJournal of Physical Chemistry B
Volume121
Issue number30
DOIs
StatePublished - Aug 3 2017

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry
  • Materials Chemistry

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