Incorporation of fucose and glucosamine into cell bound and medium released macromolecules

H. Herrmann, A. S. Havaranis, Thomas C Doetschman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Determinations were carried out on the incorporation of fucose 6 [3H] and glucosamine 6 [3H] into trichloracetic acid insoluble macromolecules which remained bound to the cells or were released into the medium of chick embryo muscle cell cultures. The radioactivity determined in the medium was corrected for unspecific binding of label to components of the medium. During an incorporation period of 6 hr the incorporation per microgram DNA with fucose as label into cell bound macromolecules is about twice as high as the incorporation into macromolecules released into medium. With glucosamine about twice as much is incorporated into medium released than into the cell bound macromolecules. The incorporation per microgram DNA increases during a culture period of 3 days but the increase ceases at different times during this culture period when determined with fucose or glucosamine or for cell bound and medium released material. An increase in cell density increases the incorporation per DNA of fucose and to a much slighter extent that of glucosamine. Reduction of cell density by addition of cytosine arabinoside to the medium does not decrease the incorporation per microgram DNA. The effect of changes of fibroblast/myoblast ratios on the incorporation of fucose and glucosamine were examined. No significant effect was observed for a ratio of 10 to 30% fibroblasts when control cultures or cultures after cell sedimentation were maintained in complete medium. Marked changes were observed after culture in medium without protein components. Under these conditions an increase in the fibroblast/myoblast ratios were observed as well as an increase in the incorporation of label into medium released and a decrease into cell bound macromolecules.

Original languageEnglish (US)
Pages (from-to)557-568
Number of pages12
JournalJournal of Cellular Physiology
Volume85
Issue number3
StatePublished - 1975
Externally publishedYes

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Fucose
Glucosamine
Macromolecules
Fibroblasts
Cell culture
Labels
Myoblasts
DNA
Cell Culture Techniques
Cell Count
Cytarabine
Radioactivity
Chick Embryo
Sedimentation
Muscle Cells
Culture Media
Muscle
Acids
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Incorporation of fucose and glucosamine into cell bound and medium released macromolecules. / Herrmann, H.; Havaranis, A. S.; Doetschman, Thomas C.

In: Journal of Cellular Physiology, Vol. 85, No. 3, 1975, p. 557-568.

Research output: Contribution to journalArticle

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