Increase of GPC Levels in Cultured Mammalian Cells during Acidosis. A 31P MR Spectroscopy Study Using a Continuous Bioreactor System

Jean‐Phillipe ‐P Galons, Constantin Job, Robert J. Gillies

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The purpose of this study was to study the metabolic events during a slow acidosis in three different cell lines by combining 31P magnetic resonance spectroscopy and hollow fiber bioreactor technology. The rate of change in intracellular pH, glycerophosphorylcholine (GPC), phophorylcholine (PCho), and nucleoside‐triphosphate (NTP) levels were measured during 8 h of acidosis and 16 h of recovery in EPO, EAT, and RN1a cells, three cultured mammalians cell lines. Our results show a significant increase in GPC levels to 330 ± 21, 540 ± 25, and 220 ± 21% of their initial value correlated to a decrease of PCho levels to 57 ± 14, 58 ± 17 and 45 ± 15% of their initial value in EAT, RN1a, and EPO cells, respectively. These changes are discussed in terms of perturbation of energetic metabolism in cells undergoing a slow acidosis.

Original languageEnglish (US)
Pages (from-to)422-426
Number of pages5
JournalMagnetic Resonance in Medicine
Volume33
Issue number3
DOIs
StatePublished - Mar 1995

Keywords

  • P MRS
  • bioreactor
  • metabolism
  • pH regulation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Increase of GPC Levels in Cultured Mammalian Cells during Acidosis. A <sup>31</sup>P MR Spectroscopy Study Using a Continuous Bioreactor System'. Together they form a unique fingerprint.

  • Cite this