Increased cyclic nucleotide phosphodiesterase activity associated with proliferation and cancer in human and murine lymphoid cells

P. M. Epstein, J. S. Mills, C. P. Ross, S. J. Strada, Evan M Hersh, W. J. Thompson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Guanosine cyclic 3'-5' monophosphate phosphodiesterase activity of murine leukemic cells (L1210) was 400 times that of human peripheral blood lymphocytes and 15 times that of murine splenic lymphocytes. Adenosine cyclic 3'-5' monophosphate (cyclic AMP) phosphodiesterase activity of L1210 cells was 320 times that of human peripheral blood lymphocytes and 24 times that of normal murine lymphocytes. The total cyclic AMP and guanosine cyclic 3'-5' monophosphate phosphodiesterase activities of rapidly growing cultured human lymphoid cells (normal and leukemic lymphoblastoid cell lines) were also markedly elevated when compared to quiescent peripheral blood lymphocytes. Human peripheral blood lymphocytes stimulated enzyme activity. Leukemic and normal murine cells contain a low affinity cyclic AMP phosphodiesterase as normally observed in most mammalian tissues. In contrast, kinetic analyses of cyclic AMP phosphodiesterase activities of human B and T lymphoblastoid cells were similar to those of peripheral blood lymphocytes in that no evidence of a low-affinity enzyme was found. All the lymphoid cells tested showed a 3 to 4 S enzyme form by linear sucrose gradient fractionation. Proliferating and quiescent human cells also contain a higher molecular weight cyclic AMP specific form (5.9 S) while the murine cells contain a 7.0 S higher molecular weight form. Both the 5.9 and 7.0 S forms show anamalous kinetic behaviour. These results are discussed with respect to the biochemical nature of cyclic nucleotide phosphodiesterases and the role of this enzyme system in the proliferation of lymphoid cells.

Original languageEnglish (US)
Pages (from-to)4016-4023
Number of pages8
JournalCancer Research
Volume37
Issue number11
StatePublished - 1977
Externally publishedYes

Fingerprint

Cyclic Nucleotides
Phosphoric Diester Hydrolases
Lymphocytes
Cyclic AMP
Neoplasms
Type 5 Cyclic Nucleotide Phosphodiesterases
Guanosine
Enzymes
Molecular Weight
Human Activities
Adenosine
Sucrose
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Epstein, P. M., Mills, J. S., Ross, C. P., Strada, S. J., Hersh, E. M., & Thompson, W. J. (1977). Increased cyclic nucleotide phosphodiesterase activity associated with proliferation and cancer in human and murine lymphoid cells. Cancer Research, 37(11), 4016-4023.

Increased cyclic nucleotide phosphodiesterase activity associated with proliferation and cancer in human and murine lymphoid cells. / Epstein, P. M.; Mills, J. S.; Ross, C. P.; Strada, S. J.; Hersh, Evan M; Thompson, W. J.

In: Cancer Research, Vol. 37, No. 11, 1977, p. 4016-4023.

Research output: Contribution to journalArticle

Epstein, P. M. ; Mills, J. S. ; Ross, C. P. ; Strada, S. J. ; Hersh, Evan M ; Thompson, W. J. / Increased cyclic nucleotide phosphodiesterase activity associated with proliferation and cancer in human and murine lymphoid cells. In: Cancer Research. 1977 ; Vol. 37, No. 11. pp. 4016-4023.
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