Increased cytosolic androgen receptor binding in rat striated muscle following denervation and disuse

P. A. Bernard, Naomi E Rance, P. S. Fishman, S. R. Max

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We investigated the effects of denervation and disuse on cytosolic androgen receptor binding by rat striated muscle. Denervation of the extensor digitorum longus and tibialis anterior muscles caused a 40-50% increase in cytosolic androgen receptor concentration with no change in apparent binding affinity. This effect was evident at 6 h postdenervation, maximal at 24 h, and declined to 120% of the control level 72 h after denervation. A 40% increase in cytosolic androgen receptor concentration was also noted 24 hr after denervation of the hormone-sensitive levator ani muscle. The effect of denervation on androgen receptors was not blocked by in vivo injection of cycloheximide; therefore, de novo receptor synthesis probably is not involved in the observed increase. Disuse, produced by subperineural injection of tetrodotoxin into the tibial and common peroneal branches of the sciatic nerve, mimicked the effect of denervation on androgen receptor binding, suggesting that neuromuscular activity is important in regulation of receptor concentration. Possible mechanisms subserving this effect are discussed.

Original languageEnglish (US)
Pages (from-to)1479-1483
Number of pages5
JournalJournal of Neurochemistry
Volume43
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

Muscle Denervation
Striated Muscle
Androgen Receptors
Denervation
Muscle
Rats
Level control
Tetrodotoxin
Muscles
Cycloheximide
Injections
Anal Canal
Sciatic Nerve
Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Increased cytosolic androgen receptor binding in rat striated muscle following denervation and disuse. / Bernard, P. A.; Rance, Naomi E; Fishman, P. S.; Max, S. R.

In: Journal of Neurochemistry, Vol. 43, No. 5, 1984, p. 1479-1483.

Research output: Contribution to journalArticle

@article{33449292c910426aa174cad5fc2c4ec7,
title = "Increased cytosolic androgen receptor binding in rat striated muscle following denervation and disuse",
abstract = "We investigated the effects of denervation and disuse on cytosolic androgen receptor binding by rat striated muscle. Denervation of the extensor digitorum longus and tibialis anterior muscles caused a 40-50{\%} increase in cytosolic androgen receptor concentration with no change in apparent binding affinity. This effect was evident at 6 h postdenervation, maximal at 24 h, and declined to 120{\%} of the control level 72 h after denervation. A 40{\%} increase in cytosolic androgen receptor concentration was also noted 24 hr after denervation of the hormone-sensitive levator ani muscle. The effect of denervation on androgen receptors was not blocked by in vivo injection of cycloheximide; therefore, de novo receptor synthesis probably is not involved in the observed increase. Disuse, produced by subperineural injection of tetrodotoxin into the tibial and common peroneal branches of the sciatic nerve, mimicked the effect of denervation on androgen receptor binding, suggesting that neuromuscular activity is important in regulation of receptor concentration. Possible mechanisms subserving this effect are discussed.",
author = "Bernard, {P. A.} and Rance, {Naomi E} and Fishman, {P. S.} and Max, {S. R.}",
year = "1984",
language = "English (US)",
volume = "43",
pages = "1479--1483",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Increased cytosolic androgen receptor binding in rat striated muscle following denervation and disuse

AU - Bernard, P. A.

AU - Rance, Naomi E

AU - Fishman, P. S.

AU - Max, S. R.

PY - 1984

Y1 - 1984

N2 - We investigated the effects of denervation and disuse on cytosolic androgen receptor binding by rat striated muscle. Denervation of the extensor digitorum longus and tibialis anterior muscles caused a 40-50% increase in cytosolic androgen receptor concentration with no change in apparent binding affinity. This effect was evident at 6 h postdenervation, maximal at 24 h, and declined to 120% of the control level 72 h after denervation. A 40% increase in cytosolic androgen receptor concentration was also noted 24 hr after denervation of the hormone-sensitive levator ani muscle. The effect of denervation on androgen receptors was not blocked by in vivo injection of cycloheximide; therefore, de novo receptor synthesis probably is not involved in the observed increase. Disuse, produced by subperineural injection of tetrodotoxin into the tibial and common peroneal branches of the sciatic nerve, mimicked the effect of denervation on androgen receptor binding, suggesting that neuromuscular activity is important in regulation of receptor concentration. Possible mechanisms subserving this effect are discussed.

AB - We investigated the effects of denervation and disuse on cytosolic androgen receptor binding by rat striated muscle. Denervation of the extensor digitorum longus and tibialis anterior muscles caused a 40-50% increase in cytosolic androgen receptor concentration with no change in apparent binding affinity. This effect was evident at 6 h postdenervation, maximal at 24 h, and declined to 120% of the control level 72 h after denervation. A 40% increase in cytosolic androgen receptor concentration was also noted 24 hr after denervation of the hormone-sensitive levator ani muscle. The effect of denervation on androgen receptors was not blocked by in vivo injection of cycloheximide; therefore, de novo receptor synthesis probably is not involved in the observed increase. Disuse, produced by subperineural injection of tetrodotoxin into the tibial and common peroneal branches of the sciatic nerve, mimicked the effect of denervation on androgen receptor binding, suggesting that neuromuscular activity is important in regulation of receptor concentration. Possible mechanisms subserving this effect are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0021686016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021686016&partnerID=8YFLogxK

M3 - Article

C2 - 6333486

AN - SCOPUS:0021686016

VL - 43

SP - 1479

EP - 1483

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 5

ER -