Increased Cytosolic Androgen Receptor Binding in Rat Striated Muscle Following Denervation and Disuse

Phyllis A. Bernard, Naomi E. Ranee, Paul S. Fishman, Stephen R. Max

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Abstract: We investigated the effects of denervation and disuse on cytosolic androgen receptor binding by rat striated muscle. Denervation of the extensor digitorum longus and tibialis anterior muscles caused a 40–50% increase in cytosolic androgen receptor concentration with no change in apparent binding affinity. This effect was evident at 6 h postdenervation, maximal at 24 h, and declined to 120% of the control level 72 h after denervation. A 40% increase in cytosolic androgen receptor concentration was also noted 24 hr after denervation of the hormone‐sensitive levator ani muscle. The effect of denervation on androgen receptors was not blocked by in vivo injection of cycloheximide; therefore, de novo receptor synthesis probably is not involved in the observed increase. Disuse, produced by subperineurial injection of tetrodotoxin into the tibial and common peroneal branches of the sciatic nerve, mimicked the effect of denervation on androgen receptor binding, suggesting that neuromuscular activity is important in regulation of receptor concentration. Possible mechanisms subserving this effect are discussed.

Original languageEnglish (US)
Pages (from-to)1479-1483
Number of pages5
JournalJournal of neurochemistry
Volume43
Issue number5
DOIs
StatePublished - Nov 1984
Externally publishedYes

Keywords

  • Androgen receptors
  • Denervation
  • Disuse
  • Muscle
  • Tetrodotoxin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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