Increased peripheral NF-κB pathway activity in women with childhood abuse-related posttraumatic stress disorder

Thaddeus Wesley Warren Pace, Katja Wingenfeld, Iris Schmidt, Gunther Meinlschmidt, Dirk H. Hellhammer, Christine M. Heim

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

In addition to neuroendocrine changes PTSD pathophysiology may also involve dysfunction of the innate immune inflammatory system. PTSD patients have been found to exhibit increased concentrations of circulating inflammatory markers such as C-reactive protein and interleukin-6, suggesting dysfunction of the innate immune inflammatory system. However, few studies have investigated molecular signaling pathways known to critically regulate inflammation. Additionally, the relationship between inflammatory function and immune cell glucocorticoid sensitivity has not been extensively explored in PTSD. Nuclear factor-κB (NF-κB) pathway activity was examined in peripheral blood mononuclear cells obtained from 12 women with childhood abuse-related PTSD and 24 healthy controls (ages 19-48) using DNA-binding ELISA. Glucocorticoid sensitivity of monocytes in whole blood was measured as the concentration of dexamethasone needed to suppress in vitro lipopolysaccharide-induced tumor necrosis factor-alpha production by 50% (DEX IC 50). Women with PTSD displayed increased NF-κB pathway activity compared to controls (t [34]=2.45, p=0.02) that was positively correlated with PTSD severity (determined by PTSD symptom severity scale) (r s=0.39, p=0.02). Increased NF-κB pathway activity was associated with increased whole blood monocyte DEX IC 50 (i.e. decreased sensitivity of monocytes to glucocorticoids) across all participants (r=0.66, p<0.001). These findings suggest that enhanced inflammatory system activity in participants with childhood abuse-related PTSD is observable at the level of NF-κB, and that in general decreased immune cell glucocorticoid sensitivity may contribute to increased NF-κB pathway activity. Enhanced inflammation may contribute to co-morbid somatic disease risk in persons with childhood abuse-related PTSD.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalBrain, Behavior, and Immunity
Volume26
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Fingerprint

Post-Traumatic Stress Disorders
Glucocorticoids
Monocytes
Immune System
Inflammation
C-Reactive Protein
Dexamethasone
Lipopolysaccharides
Interleukin-6
Blood Cells
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
DNA

Keywords

  • Early life stress
  • Glucocorticoid sensitivity
  • Glucocorticoid-immune interactions
  • Inflammation
  • Inflammatory signaling
  • PTSD

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Increased peripheral NF-κB pathway activity in women with childhood abuse-related posttraumatic stress disorder. / Pace, Thaddeus Wesley Warren; Wingenfeld, Katja; Schmidt, Iris; Meinlschmidt, Gunther; Hellhammer, Dirk H.; Heim, Christine M.

In: Brain, Behavior, and Immunity, Vol. 26, No. 1, 01.2012, p. 13-17.

Research output: Contribution to journalArticle

Pace, Thaddeus Wesley Warren ; Wingenfeld, Katja ; Schmidt, Iris ; Meinlschmidt, Gunther ; Hellhammer, Dirk H. ; Heim, Christine M. / Increased peripheral NF-κB pathway activity in women with childhood abuse-related posttraumatic stress disorder. In: Brain, Behavior, and Immunity. 2012 ; Vol. 26, No. 1. pp. 13-17.
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