Increased platelet cytosolic calcium responses to low density lipoprotein in type ii diabetes with and without hypertension

Paul R. Standley, Safdar Am, Chandra Bapna, James R. Sowers

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Non-insulin dependent diabetes mellitus (NIDDM) and hypertension are common diseases which are independently associated with insulin resistance/hyperinsulinemia, dyslysidemia, abnormalities of platelet function, and accelerated atherogenesis. The interaction of these independent risk factors is poorly understood. Recently, low density lipoprotein (LDL) receptors have been described, in platelets, and LDL elevates [Ca2+]i in these cells. In this study we have evaluated platelet [Ca2+]i responsiveness to LDL and arginine vasopressin (AVP) in NIDDM patients with (n = 28) and without (n = 13) concomitant hypertension, as well as in normal nondiabetic controls (n = 13). Platelet [Ca2+]i concentration-response curves to LDL for both NIDDM and hypertensive NIDDM were shifted significantly to the left when compared to the nor- motensive, nondiabetic controls. By contrast, no differences were seen in [Ca2+li responses to 10 pmol/L A VP among any of the groups. To determine the possible role of hyperinsulinemia in this accentuated [Ca2+li response to LDL, we measured basal and LDL-stimulated [Ca2+li in platelets of normal volunteers after insulin treatment (0-100 mU/mL for 30 and 90 min). Insulin did not alter baseline or LDL-stimulated (150 mg/mL) platelet [Ca2+li. Thus, an enhanced platelet [Ca2+li response to LDL is characteristic of diabetes, independently of blood pressure. As such, it may also help to explain the enhanced platelet aggregation, endothelial dysfunction, and accelerated atherosclerosis of NIDDM. Am J Hypertens 1993;6:938- 943.

Original languageEnglish (US)
Pages (from-to)938-943
Number of pages6
JournalAmerican Journal of Hypertension
Volume6
Issue number11
DOIs
StatePublished - Nov 1993
Externally publishedYes

Keywords

  • Arginine vasopressin
  • Hypertension
  • Low density lipoprotein receptors
  • Non-insulin dependent diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine

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