Increased thermoregulation in cold-exposed transgenic mice overexpressing lipoprotein lipase in skeletal muscle

An avian phenotype?

Dalan R. Jensen, Leslie A. Knaub, John Konhilas, Leslie A. Leinwand, Paul S. MacLean, Robert H. Eckel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

LPL is an enzyme involved in the breakdown and uptake of lipoprotein triglycerides. In the present study, we examined how the transgenic (Tg) overexpression of human LPL in mouse skeletal muscle affected tolerance to cold temperatures, cold-induced thermogenesis, and fuel utilization during this response. Tg mice and their non-transgenic controls were placed in an environmental chamber and housed in metabolic chambers that monitored oxygen consumption and carbon dioxide production with calorimetry. When exposed to 4°C, an attenuation in the decline in body temperature in Tg mice was accompanied by an increased metabolic rate (15%; P < 0.001) and a reduction in respiratory quotient (P < 0.05). Activity levels, the expression of uncoupling proteins in brown fat and muscle, and lean mass failed to explain the enhanced cold tolerance and thermogenesis in Tg mice. The more oxida- tive type IIa fibers were favored over the more glycolytic type IIb fibers ( P < 0.001) in the gastrocnemius and quadriceps muscles of Tg mice.

Original languageEnglish (US)
Pages (from-to)870-879
Number of pages10
JournalJournal of Lipid Research
Volume49
Issue number4
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

Fingerprint

Lipoprotein Lipase
Body Temperature Regulation
Transgenic Mice
Muscle
Skeletal Muscle
Phenotype
Thermogenesis
Environmental chambers
Fibers
Calorimetry
Carbon Dioxide
Brown Adipose Tissue
Quadriceps Muscle
Body Temperature
Fats
Oxygen Consumption
Oxygen
Temperature
Enzymes
Muscles

Keywords

  • Activity
  • Birds
  • Body temperature
  • Fiber-typing
  • Metabolic rate
  • Respiratory quotient
  • Uncoupling proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Increased thermoregulation in cold-exposed transgenic mice overexpressing lipoprotein lipase in skeletal muscle : An avian phenotype? / Jensen, Dalan R.; Knaub, Leslie A.; Konhilas, John; Leinwand, Leslie A.; MacLean, Paul S.; Eckel, Robert H.

In: Journal of Lipid Research, Vol. 49, No. 4, 01.04.2008, p. 870-879.

Research output: Contribution to journalArticle

Jensen, Dalan R. ; Knaub, Leslie A. ; Konhilas, John ; Leinwand, Leslie A. ; MacLean, Paul S. ; Eckel, Robert H. / Increased thermoregulation in cold-exposed transgenic mice overexpressing lipoprotein lipase in skeletal muscle : An avian phenotype?. In: Journal of Lipid Research. 2008 ; Vol. 49, No. 4. pp. 870-879.
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