Increased vacuolar and plasma membrane H+-ATPase activities in Salicornia bigelovii Torr. in response to NaCl

Felix Ayala, James W. O'Leary, Karen S Schumaker

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The halophyte Salicornia bigelovii Torr, shows optimal growth and Na+ accumulation in 200mM NaCl and reduced growth under lower salinity conditions. The ability to accumulate and compartmentalize Na+ may result, in part, from stimulation of the H+-ATPases on the plasma membrane (PM-ATPase) and vacuolar membranes (V-ATPase). To determine if these two primary transport systems are involved in salt tolerance, shoot fresh weight (FW) and activity of the PM- and V-ATPases from shoots in Salicornia grown in 5 and 200 mM NaCl were compared. Higher PM-ATPase activity (60%) and FW (60%) were observed in plants grown in 200 mM NaCl and these stimulations in growth and enzyme activity were specific for Na+ and not observed with Na+ added in vitro. V-ATPase activity was significantly stimulated in vivo and in vitro (26% and 46%, respectively) after exposure to 200 mM NaCl, and stimulation was Na+-specific, Immunoblots indicated that the increases in activity of the H+-ATPases from plants grown in 200 mM NaCI was not due to increases in protein expression. These studies suggest that the H+-ATPases in Salicornia are important in salt tolerance and provide a biochemical framework for understanding mechanisms of salt tolerance in plants.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalJournal of Experimental Botany
Volume47
Issue number294
StatePublished - Jan 1996

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Salicornia bigelovii
Chenopodiaceae
Vacuolar Proton-Translocating ATPases
H-transporting ATP synthase
Salt-Tolerance
Proton-Translocating ATPases
Adenosine Triphosphatases
plasma membrane
Cell Membrane
salt tolerance
Salicornia
Growth
Weights and Measures
adenosinetriphosphatase
Salinity
shoots
Membranes
Enzymes
protein synthesis
enzyme activity

Keywords

  • H-ATPases
  • Salicornia
  • Salt tolerance

ASJC Scopus subject areas

  • Plant Science

Cite this

Increased vacuolar and plasma membrane H+-ATPase activities in Salicornia bigelovii Torr. in response to NaCl. / Ayala, Felix; O'Leary, James W.; Schumaker, Karen S.

In: Journal of Experimental Botany, Vol. 47, No. 294, 01.1996, p. 25-32.

Research output: Contribution to journalArticle

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