Increases in VO2max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats

Dileep S. Sachan, Nobuko Hongu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have previously shown that the combination of caffeine, carnitine, and choline supplementation decreased body fat and serum leptin concentration in rats and was attributed to increased fat utilization for energy. As a result, it was hypothesized that the supplements may augment exercise performance including physiological and biochemical indexes. Twenty 7-week-old male Sprague-Dawley rats were given free access to a nonpurified diet with or without supplementation of caffeine, carnitine, and choline at concentrations of 0.1, 5, and 11.5 g/kg diet, respectively. One half of each dietary group was exercised on a motor-driven treadmill for 3 weeks and maximal aerobic power (VO2max) was determined on the 18th day of exercise. Rats were killed 24-hr postexercise, and blood, regional fat pads, and skeletal muscle were collected. The VO2max was increased (P < 0.05) in the supplemented/exercised group; however, the respiratory quotient (RQ) was not affected. Postexercised concentrations of serum triglycerides were decreased but β-hydroxybutyrate, acylcarnitine, and acetylcarnitine were increased in the supplemented animals. The changes in serum metabolites were complemented by the changes in the muscle and urinary metabolites. The magnitude of increase in urinary acylcarnitines (34-45-fold) is a unique effect of this combination of supplements. Cumulative evidence indicates enhanced β-oxidation of fatty acids without a change in the RQ because acetyl units were excreted in urine as acetylcarnitine and not oxidized to carbon dioxide. For this phenomenon, we propose the term 'fatty acid dumping.' We conclude that supplementation with caffeine, carnitine, and choline augments exercise performance and promotes fatty acid oxidation as well as disposal in urine. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)521-526
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume11
Issue number10
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Carnitine
Choline
Caffeine
Acetylcarnitine
Rats
Fatty Acids
Fats
Nutrition
Metabolites
Oxidation
Muscle
Adipose Tissue
Serum
Urine
Hydroxybutyrates
Diet
Exercise equipment
Leptin
Carbon Dioxide
Sprague Dawley Rats

Keywords

  • Caffeine
  • Carnitine
  • Choline
  • Exercise
  • Fat oxidation
  • Rats
  • VOmax

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Increases in VO2max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats. / Sachan, Dileep S.; Hongu, Nobuko.

In: Journal of Nutritional Biochemistry, Vol. 11, No. 10, 2000, p. 521-526.

Research output: Contribution to journalArticle

@article{942e93f83cab447cb68f34e0b387b945,
title = "Increases in VO2max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats",
abstract = "We have previously shown that the combination of caffeine, carnitine, and choline supplementation decreased body fat and serum leptin concentration in rats and was attributed to increased fat utilization for energy. As a result, it was hypothesized that the supplements may augment exercise performance including physiological and biochemical indexes. Twenty 7-week-old male Sprague-Dawley rats were given free access to a nonpurified diet with or without supplementation of caffeine, carnitine, and choline at concentrations of 0.1, 5, and 11.5 g/kg diet, respectively. One half of each dietary group was exercised on a motor-driven treadmill for 3 weeks and maximal aerobic power (VO2max) was determined on the 18th day of exercise. Rats were killed 24-hr postexercise, and blood, regional fat pads, and skeletal muscle were collected. The VO2max was increased (P < 0.05) in the supplemented/exercised group; however, the respiratory quotient (RQ) was not affected. Postexercised concentrations of serum triglycerides were decreased but β-hydroxybutyrate, acylcarnitine, and acetylcarnitine were increased in the supplemented animals. The changes in serum metabolites were complemented by the changes in the muscle and urinary metabolites. The magnitude of increase in urinary acylcarnitines (34-45-fold) is a unique effect of this combination of supplements. Cumulative evidence indicates enhanced β-oxidation of fatty acids without a change in the RQ because acetyl units were excreted in urine as acetylcarnitine and not oxidized to carbon dioxide. For this phenomenon, we propose the term 'fatty acid dumping.' We conclude that supplementation with caffeine, carnitine, and choline augments exercise performance and promotes fatty acid oxidation as well as disposal in urine. Copyright (C) 2000 Elsevier Science Inc.",
keywords = "Caffeine, Carnitine, Choline, Exercise, Fat oxidation, Rats, VOmax",
author = "Sachan, {Dileep S.} and Nobuko Hongu",
year = "2000",
doi = "10.1016/S0955-2863(00)00119-4",
language = "English (US)",
volume = "11",
pages = "521--526",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Increases in VO2max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats

AU - Sachan, Dileep S.

AU - Hongu, Nobuko

PY - 2000

Y1 - 2000

N2 - We have previously shown that the combination of caffeine, carnitine, and choline supplementation decreased body fat and serum leptin concentration in rats and was attributed to increased fat utilization for energy. As a result, it was hypothesized that the supplements may augment exercise performance including physiological and biochemical indexes. Twenty 7-week-old male Sprague-Dawley rats were given free access to a nonpurified diet with or without supplementation of caffeine, carnitine, and choline at concentrations of 0.1, 5, and 11.5 g/kg diet, respectively. One half of each dietary group was exercised on a motor-driven treadmill for 3 weeks and maximal aerobic power (VO2max) was determined on the 18th day of exercise. Rats were killed 24-hr postexercise, and blood, regional fat pads, and skeletal muscle were collected. The VO2max was increased (P < 0.05) in the supplemented/exercised group; however, the respiratory quotient (RQ) was not affected. Postexercised concentrations of serum triglycerides were decreased but β-hydroxybutyrate, acylcarnitine, and acetylcarnitine were increased in the supplemented animals. The changes in serum metabolites were complemented by the changes in the muscle and urinary metabolites. The magnitude of increase in urinary acylcarnitines (34-45-fold) is a unique effect of this combination of supplements. Cumulative evidence indicates enhanced β-oxidation of fatty acids without a change in the RQ because acetyl units were excreted in urine as acetylcarnitine and not oxidized to carbon dioxide. For this phenomenon, we propose the term 'fatty acid dumping.' We conclude that supplementation with caffeine, carnitine, and choline augments exercise performance and promotes fatty acid oxidation as well as disposal in urine. Copyright (C) 2000 Elsevier Science Inc.

AB - We have previously shown that the combination of caffeine, carnitine, and choline supplementation decreased body fat and serum leptin concentration in rats and was attributed to increased fat utilization for energy. As a result, it was hypothesized that the supplements may augment exercise performance including physiological and biochemical indexes. Twenty 7-week-old male Sprague-Dawley rats were given free access to a nonpurified diet with or without supplementation of caffeine, carnitine, and choline at concentrations of 0.1, 5, and 11.5 g/kg diet, respectively. One half of each dietary group was exercised on a motor-driven treadmill for 3 weeks and maximal aerobic power (VO2max) was determined on the 18th day of exercise. Rats were killed 24-hr postexercise, and blood, regional fat pads, and skeletal muscle were collected. The VO2max was increased (P < 0.05) in the supplemented/exercised group; however, the respiratory quotient (RQ) was not affected. Postexercised concentrations of serum triglycerides were decreased but β-hydroxybutyrate, acylcarnitine, and acetylcarnitine were increased in the supplemented animals. The changes in serum metabolites were complemented by the changes in the muscle and urinary metabolites. The magnitude of increase in urinary acylcarnitines (34-45-fold) is a unique effect of this combination of supplements. Cumulative evidence indicates enhanced β-oxidation of fatty acids without a change in the RQ because acetyl units were excreted in urine as acetylcarnitine and not oxidized to carbon dioxide. For this phenomenon, we propose the term 'fatty acid dumping.' We conclude that supplementation with caffeine, carnitine, and choline augments exercise performance and promotes fatty acid oxidation as well as disposal in urine. Copyright (C) 2000 Elsevier Science Inc.

KW - Caffeine

KW - Carnitine

KW - Choline

KW - Exercise

KW - Fat oxidation

KW - Rats

KW - VOmax

UR - http://www.scopus.com/inward/record.url?scp=0033663129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033663129&partnerID=8YFLogxK

U2 - 10.1016/S0955-2863(00)00119-4

DO - 10.1016/S0955-2863(00)00119-4

M3 - Article

VL - 11

SP - 521

EP - 526

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

IS - 10

ER -