Increasing ligninolytic enzyme activities in several white-rot Basidiomycetes by nitrogen-sufficient media

Erwin E J Kaal, James A Field, Thomas W. Joyce

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Ligninolytic enzyme activities were monitored in five white-rot fungi cultivated on nitrogen (N)-limited glucose-BIII medium and were compared with the activities obtained in media supplemented with 56 mm peptone-N. Only Phanerochaete chrysosporium and two Bjerkandera strains produced detectable lignin peroxidase (LiP) activity. LiP was stimulated by either N-limitation or N-sufficiency in P. chrysosporium and Bjerkandera spp., respectively. All of the fungal strains tested produced manganese-dependent peroxidase (MnP) activity, which was consistently stimulated by the peptone supplementation. Both the manganese-independent peroxidase (MiP) activities, which were detected only in Bjerkandera spp., and the laccase activities, which were detected only in Lentinula edodes and Pleurotus ostreatus, were also enhanced by peptone. In several fungal strains, the activity of ligninolytic enzymes was likewise stimulated by supplying 56 mm NH+4-N at an initial pH of 7·3. This study indicates that several commercially important and commonly occurring white-rot fungi produce higher ligninolytic enzyme activities in response to a nitrogen-rich medium, in contrast to the physiological model proposed for P. chrysosporium.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalBioresource Technology
Volume53
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Bjerkandera
Coriolaceae
manganese peroxidase
Phanerochaete
Phanerochaete chrysosporium
Basidiomycota
Peptones
peptones
Enzyme activity
lignin peroxidase
lignin
enzyme activity
manganese
white-rot fungi
Nitrogen
fungus
Lignin
Fungi
Manganese
nitrogen

Keywords

  • Bjerkandera adusta
  • laccase
  • Lentinula edodes
  • lignin peroxidase
  • manganese-dependent peroxidase
  • Phanerochaete chrysosporium
  • Pleurotus ostreatus
  • White-rot fungi

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal
  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Increasing ligninolytic enzyme activities in several white-rot Basidiomycetes by nitrogen-sufficient media. / Kaal, Erwin E J; Field, James A; Joyce, Thomas W.

In: Bioresource Technology, Vol. 53, No. 2, 1995, p. 133-139.

Research output: Contribution to journalArticle

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AB - Ligninolytic enzyme activities were monitored in five white-rot fungi cultivated on nitrogen (N)-limited glucose-BIII medium and were compared with the activities obtained in media supplemented with 56 mm peptone-N. Only Phanerochaete chrysosporium and two Bjerkandera strains produced detectable lignin peroxidase (LiP) activity. LiP was stimulated by either N-limitation or N-sufficiency in P. chrysosporium and Bjerkandera spp., respectively. All of the fungal strains tested produced manganese-dependent peroxidase (MnP) activity, which was consistently stimulated by the peptone supplementation. Both the manganese-independent peroxidase (MiP) activities, which were detected only in Bjerkandera spp., and the laccase activities, which were detected only in Lentinula edodes and Pleurotus ostreatus, were also enhanced by peptone. In several fungal strains, the activity of ligninolytic enzymes was likewise stimulated by supplying 56 mm NH+4-N at an initial pH of 7·3. This study indicates that several commercially important and commonly occurring white-rot fungi produce higher ligninolytic enzyme activities in response to a nitrogen-rich medium, in contrast to the physiological model proposed for P. chrysosporium.

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