Increasing transduction efficiency by lentivirus vectors using heat shock and cell cycle specific integration

Michael Badowski, David T. Harris

Research output: Contribution to journalArticle

Abstract

Gene therapy has evolved over the past 20 years and has seen both great success and astounding failure. Lentivirus vectors have been shown to be powerful tools for gene therapy that can deliver a transgene to an extremely wide variety of cell types. However, use of these vectors still carries risks of insertional mutagenesis and viral toxicity. Safety can be increased if we can, in any given circumstance, use fewer viral particles or less overall viral protein. Therefore, strategies that increase efficiency of viral transduction will affect an increase in safety. Both cell cycle timing and heat shock have been used to increase the efficiency of various methods of gene delivery. We show that combining these techniques offers greater transduction efficiency than either technique alone, due to cell cycle specific integration that is observed as increased transgene expression.

Original languageEnglish (US)
Pages (from-to)284-293
Number of pages10
JournalGene Therapy and Molecular Biology
Volume13
Issue number2
StatePublished - 2009

Fingerprint

Lentivirus
Transgenes
Genetic Therapy
Shock
Cell Cycle
Hot Temperature
Safety
Insertional Mutagenesis
Viral Proteins
Virion
Genes

Keywords

  • Cell cycle
  • Genistein
  • Heat shock
  • Lentivirus vector

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine

Cite this

Increasing transduction efficiency by lentivirus vectors using heat shock and cell cycle specific integration. / Badowski, Michael; Harris, David T.

In: Gene Therapy and Molecular Biology, Vol. 13, No. 2, 2009, p. 284-293.

Research output: Contribution to journalArticle

@article{b645d3b6a30e4911953177587b0f2253,
title = "Increasing transduction efficiency by lentivirus vectors using heat shock and cell cycle specific integration",
abstract = "Gene therapy has evolved over the past 20 years and has seen both great success and astounding failure. Lentivirus vectors have been shown to be powerful tools for gene therapy that can deliver a transgene to an extremely wide variety of cell types. However, use of these vectors still carries risks of insertional mutagenesis and viral toxicity. Safety can be increased if we can, in any given circumstance, use fewer viral particles or less overall viral protein. Therefore, strategies that increase efficiency of viral transduction will affect an increase in safety. Both cell cycle timing and heat shock have been used to increase the efficiency of various methods of gene delivery. We show that combining these techniques offers greater transduction efficiency than either technique alone, due to cell cycle specific integration that is observed as increased transgene expression.",
keywords = "Cell cycle, Genistein, Heat shock, Lentivirus vector",
author = "Michael Badowski and Harris, {David T.}",
year = "2009",
language = "English (US)",
volume = "13",
pages = "284--293",
journal = "Gene Therapy and Molecular Biology",
issn = "1529-9120",
publisher = "Gene Therapy and Molecular Biology",
number = "2",

}

TY - JOUR

T1 - Increasing transduction efficiency by lentivirus vectors using heat shock and cell cycle specific integration

AU - Badowski, Michael

AU - Harris, David T.

PY - 2009

Y1 - 2009

N2 - Gene therapy has evolved over the past 20 years and has seen both great success and astounding failure. Lentivirus vectors have been shown to be powerful tools for gene therapy that can deliver a transgene to an extremely wide variety of cell types. However, use of these vectors still carries risks of insertional mutagenesis and viral toxicity. Safety can be increased if we can, in any given circumstance, use fewer viral particles or less overall viral protein. Therefore, strategies that increase efficiency of viral transduction will affect an increase in safety. Both cell cycle timing and heat shock have been used to increase the efficiency of various methods of gene delivery. We show that combining these techniques offers greater transduction efficiency than either technique alone, due to cell cycle specific integration that is observed as increased transgene expression.

AB - Gene therapy has evolved over the past 20 years and has seen both great success and astounding failure. Lentivirus vectors have been shown to be powerful tools for gene therapy that can deliver a transgene to an extremely wide variety of cell types. However, use of these vectors still carries risks of insertional mutagenesis and viral toxicity. Safety can be increased if we can, in any given circumstance, use fewer viral particles or less overall viral protein. Therefore, strategies that increase efficiency of viral transduction will affect an increase in safety. Both cell cycle timing and heat shock have been used to increase the efficiency of various methods of gene delivery. We show that combining these techniques offers greater transduction efficiency than either technique alone, due to cell cycle specific integration that is observed as increased transgene expression.

KW - Cell cycle

KW - Genistein

KW - Heat shock

KW - Lentivirus vector

UR - http://www.scopus.com/inward/record.url?scp=77649276654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649276654&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 284

EP - 293

JO - Gene Therapy and Molecular Biology

JF - Gene Therapy and Molecular Biology

SN - 1529-9120

IS - 2

ER -