Independent expression of cardiac type I and II cyclic AMP-dependent protein kinase during murine embryogenesis and postnatal development

Mari K. Haddox, William R. Roeske, Diane Haddock Russell

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The amount of total cyclic AMP-dependent protein kinase and of the protein kinase isozymes present in mouse heart changes during development. During embryogenesis, the total cardiac protein kinase activity increases most markedly during the 6 days prior to birth. A maximum kinase level is achieved in the 7 day-old neonate, and then activity progressively declines to an adult level approximating that of the mid-embryo. The type II kinase exhibits a moderate increase during late embryogenesis which declines by the time of birth. The type I isozyme increases throughout embryogenesis and the first neonatal week to a maximum specific activity five-fold higher than the mid-embryogenesis level. The isozyme level then falls to an adult activity similar to the mid-embryonic. These changes in isozyme profile are reflected in a changing type I to type II kinase ratio of 1.1 at 13-14 days embryogenesis, 2.4 at birth, 3.0 in the 7 day-old neonates, and 1 in the adult heart. Thus, the two protein kinase isozymes change in association with the developmental process in an independent fashion.

Original languageEnglish (US)
Pages (from-to)527-534
Number of pages8
JournalBBA - General Subjects
Volume585
Issue number4
DOIs
StatePublished - Jul 18 1979
Externally publishedYes

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Keywords

  • (Murine)
  • Cycle AMP
  • Embryogenesis
  • Protein kinase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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