Peroxisome assembly in Saccharomyces cerevisiae requires the products of several genes. In this report, the PAS5 gene has been characterized. The gene is on the left arm of chromosome X and encodes a polypeptide with similarity to the mammalian peroxisome assembly factor-1 (PAF-1). Two different length transcripts are produced from the yeast PAS5 gene. The longer mRNAs encompass an open reading frame, while the shorter transcripts initiate 46-110 base pairs downstream of the first in-frame AUG. The longer transcripts are induced four-fold on medium containing fatty acids as the sole carbon source, while the shorter transcripts are induced up to ten-fold on medium containing glycerol as a carbon source. The full-length coding sequence encodes a protein with a calculated molecular weight of 30-7 kDa. A protein of 25 kDa could be translated from the shorter transcripts and would lack a very acidic domain found in the ammo-terminal extension of the longer protein. The common portion of the proteins is very basic; the calculated pi of the longer polypeptide is 9-02 and that of the shorter protein is 10-06. The PAS5 GenBank accession number is M86538.
|Original language||English (US)|
|Number of pages||9|
|State||Published - Feb 1996|
- Zellweger syndrome
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology