Induced Neural Stem Cells Generated from Rat Fibroblasts

Guangjun Xi, Pingfang Hu, Cunye Qu, Shenfeng Qiu, Chang Tong, Qi Long Ying

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

Original languageEnglish (US)
Pages (from-to)312-319
Number of pages8
JournalGenomics, Proteomics and Bioinformatics
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2013
Externally publishedYes

Keywords

  • Fibroblasts
  • Induced neural stem cells
  • Induced pluripotent stem cells
  • Rat
  • Stem cell self-renewal

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Computational Mathematics

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