Induction of DNA strand breaks and DNA-protein cross-links in normal human skin fibroblasts following exposure to 254 nm UV radiation

Li Wen Lai, Barry S. Rosenstein

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Levels of DNA strand breaks and DNA-protein cross-links (DPCs) were measured using the alkaline elution assay in normal human skin fibroblasts irradiated with 0-200 J m-2 of 254 nm UV radiation and incubated for 0-24 h. On incubation, the yields of both single-strand breaks (SSBs) and DPCs increased with similar kinetics and remained elevated. In addition, when SSBs were measured under conditions in which DPCs were not eliminated by treatment with proteinase K, a measurable yield of SSBs could not be detected. Hence, the SSBs that form in the UV-irradiated cells following incubation appear to be associated with the DPCs.

Original languageEnglish (US)
Pages (from-to)395-404
Number of pages10
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume6
Issue number4
DOIs
StatePublished - Aug 1990

Keywords

  • 254 nm UV
  • DNA strand breakage
  • DNA-protein cross-linking
  • human skin cells.

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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