Induction of interleukin-4-dependent IgE synthesis and interleukin-5-dependent eosinophil differentiation by supernatants of a human helper T-cell clone

Haifa H. Jabara, Steven J. Ackerman, Donata Vercelli, Takashi Yokota, Ken Ichi Arai, John Abrams, Ann M. Dvorak, Mark C. Lavigne, Jacques Banchereau, Jan de Vries, Donald Y.M. Leung, Raif S. Geha

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The human alloreactive T-cell clone A1 produces interleukin (IL)-4, IL-5, and granulocyte macrophage-colony stimulating factor (GM-CSF), but not IL-2 or interferon-γ (IFN-γ), as assessed by probing for mRNA transcripts, immunoassays, or bioassays. Supernatants from clone A1 induced IgE synthesis by normal human peripheral blood mononuclear cells. IL-4 was shown to be necessary and sufficient for the induction of IgE by clone A1 supernatants. In contrast, IgE induction by clone A1 supernatants and recombinant (r) IL-4 was inhibited by IFN-γ. This suggests that the absence of IFN-γ from the IL-4-containing A1 supernatants was important for their IgE-inducing ability. Supernatants from clone A1 could also specifically induce the growth of cord blood cell progenitors and their differentiation into eosinophils but not into basophils. rIL-5, but not rIL-4 or rGM-CSF, also induced eosinophil maturation. These data suggest that IL-5 secreted by clone A1 was responsible for its ability to induce eosinophil differentiation. The implications of the concomitant production of IL-4 and IL-5 by a single T-cell clone are discussed.

Original languageEnglish (US)
Pages (from-to)437-446
Number of pages10
JournalJournal of Clinical Immunology
Volume8
Issue number6
DOIs
StatePublished - Nov 1988
Externally publishedYes

Keywords

  • IL-5
  • IgE synthesis
  • Interleukin (IL)-4
  • eosinophil differentiation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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